Kepron W, James J M, Kirk B, Sehon A H, Tse K S
J Allergy Clin Immunol. 1977 Jan;59(1):64-9. doi: 10.1016/0091-6749(77)90178-6.
Immunization of neonatal dogs with a conjugate of 2,4-dinitrobenzene and ovalbumin (DNP2-OA), using aluminum hydroxide as the adjuvant, elicited long-lasting (over 30 wk) anti-DNP and anti-OA IgE antibody responses of high titers as determined by homologous passive cutaneous anaphylaxis. Low antigen doses of 10 or 50 mug were more effective than the higher doses of 250 or 1,250 mug in inducing high IgE antibody levels. However, this method of immunization failed to elicity any detectable IgE antibody response in adult dogs. Bronchoprovocation with antigen of sensitized animals having IgE antibody titers in excess of 64 resulted in a marked increase in airflow resistance, which could be corrected by the administration of nebulized isoproterenol. On the other hand, sensitized animals with IgE antibody titers in the order of 64 did not manifest significant bronchoconstriction on inhalation challenge but developed anaphylaxis following intravenous injection of the antigen.
以氢氧化铝为佐剂，用2,4 -二硝基苯与卵清蛋白的偶联物（DNP2 - OA）对新生犬进行免疫接种，通过同源被动皮肤过敏反应测定，引发了高滴度的持久（超过30周）抗DNP和抗OA IgE抗体反应。低抗原剂量10或50微克比高剂量250或1250微克在诱导高IgE抗体水平方面更有效。然而，这种免疫方法未能在成年犬中引发任何可检测到的IgE抗体反应。对IgE抗体滴度超过64的致敏动物进行抗原激发试验，气流阻力显著增加，雾化吸入异丙肾上腺素可纠正这一情况。另一方面，IgE抗体滴度约为64的致敏动物在吸入激发试验中未表现出明显的支气管收缩，但静脉注射抗原后会发生过敏反应。
