Pousette A, Akerlöf E, Lundin A, Rosenborg L, Fredricsson B
Int J Androl. 1986 Oct;9(5):331-40. doi: 10.1111/j.1365-2605.1986.tb00895.x.
Following the development of a reliable technique for analysis of total ATP in semen, the ATP concentration of untreated human ejaculates was compared with progressive sperm motility during 3 continuous days following ejaculation. As correlation was poor between these variables, spermatozoa were separated by self-migration on Percoll gradients and washed free of Percoll solution. ATP concentration and sperm motility was then assessed. Aliquots of separated sperms were transferred to separate tubes, and increasing concentrations of sera were added prior to incubation at 37 degrees C for 4 days. Under these conditions a good correlation between specific progressive motility and specific ATP content was found (r = 0.88). A high concentration of human blood serum (10% v/v) was found to be necessary for maintainance of motility, but neither the addition of bovine serum albumin nor an increase in the glucose concentration had any effect on sperm motility or on the ATP concentration. Addition of different fractions of human blood serum indicated that the components mainly responsible for maintaining sperm motility are greater than 10,000 daltons. Our findings strongly indicate that ATP assessment can be used to estimate the energetic condition of a human sperm population after its separation from seminal plasma. It is suggested that this in vitro system can be used to define optimal conditions for sperm function.
在开发出一种可靠的精液总ATP分析技术后，将未处理的人类射精样本的ATP浓度与射精后连续3天的精子渐进性运动能力进行了比较。由于这些变量之间的相关性较差，精子通过在Percoll梯度上的自我迁移进行分离，并洗去Percoll溶液。然后评估ATP浓度和精子运动能力。将分离出的精子等分试样转移到单独的试管中，并在37℃孵育4天之前加入浓度递增的血清。在这些条件下，发现特定的渐进性运动能力与特定的ATP含量之间存在良好的相关性（r = 0.88）。发现高浓度的人血清（10% v/v）对于维持运动能力是必要的，但添加牛血清白蛋白或增加葡萄糖浓度对精子运动能力或ATP浓度均无任何影响。添加不同组分的人血清表明，主要负责维持精子运动能力的成分分子量大于10,000道尔顿。我们的研究结果强烈表明，ATP评估可用于估计人类精子群体从精浆中分离后的能量状态。建议该体外系统可用于确定精子功能的最佳条件。
