用于分离人类精子的上游法和Percoll梯度离心法的比较。

Comparison between a swim-up and a Percoll gradient technique for the separation of human spermatozoa.

作者信息

Akerlöf E, Fredricson B, Gustafsson O, Lundin A, Lunell N O, Nylund L, Rosenborg L, Pousette A

机构信息

Department of Clinical Chemistry, Karolinska Institute, Huddinge University Hospital, Sweden.

出版信息

Int J Androl. 1987 Oct;10(5):663-9. doi: 10.1111/j.1365-2605.1987.tb00367.x.

DOI:10.1111/j.1365-2605.1987.tb00367.x

PMID:3319916

Abstract

Two methods of separating human sperm were compared using twenty-two semen samples. The sperm were separated by a swim-up technique or by self-migration on a Percoll gradient followed by medium change. After separation, the sperm obtained were assessed for progressive motility, ATP content, energy charge index ([ATP + 0.5 ADP]/[ATP + ADP + AMP]) and morphology. In general, and especially for semen samples containing less than 20 X 10(6) sperm/ml, separation by Percoll gradient selected sperm that were superior to those separated by the swim-up technique. The relatively high energy charge index (greater than 0.8) showed that the sperm tolerated the separation conditions well. It is suggested that self-migration on a Percoll gradient should prove useful for obtaining sperm of high quality.

摘要

采用22份精液样本对两种分离人类精子的方法进行了比较。精子通过上游技术或在Percoll梯度上自行迁移并随后更换培养基进行分离。分离后，对获得的精子进行了活力、ATP含量、能量电荷指数（[ATP + 0.5 ADP]/[ATP + ADP + AMP]）和形态学评估。总体而言，尤其是对于精子浓度低于20×10⁶/ml的精液样本，通过Percoll梯度分离出的精子优于通过上游技术分离出的精子。相对较高的能量电荷指数（大于0.8）表明精子对分离条件耐受性良好。建议Percoll梯度上的自行迁移对于获取高质量精子应是有用的。