Normand Philippe, Pujic Petar, Abrouk Danis, Vemulapally Spandana, Guerra Trina, Carlos-Shanley Camila, Hahn Dittmar
Université Claude-Bernard Lyon 1, Université de Lyon, UMR 5557 CNRS Ecologie Microbienne, Villeurbanne, France.
Texas State University, Department of Biology, 601 University Drive, San Marcos, TX 78666, USA.
J Genomics. 2022 Jun 6;10:49-56. doi: 10.7150/jgen.74788. eCollection 2022.
The genomes of two nitrogen-fixing strains, Ag45/Mut15 and AgPM24, isolated from root nodules of are described as representatives of a novel candidate species Phylogenomic and ANI analyses confirmed that both strains are related to cluster 1 frankiae, and that both strains belong to a novel species. At 6.4 - 6.7 Mb, their genomes were smaller than those of other cultivated -infective cluster 1 strains but larger than that of the non-cultivated -infective cluster 1 Sp+ strain AgTrS that was their closest neighbor as assessed by ANI. Comparative genomic analyses identified genes essential for nitrogen-fixation, gene composition as regards COGs, secondary metabolites clusters and transcriptional regulators typical of those from -infective cluster 1 cultivated strains in both genomes. There were 459 genes present in other cultivated -infective strains lost in the two genomes, spread over the whole of the genome, which indicates genome erosion is taking place in these two strains.
从根瘤中分离出的两株固氮菌株Ag45/Mut15和AgPM24的基因组，被描述为一个新候选物种的代表。系统基因组学和平均核苷酸一致性（ANI）分析证实，这两株菌株均与簇1弗兰克氏菌相关，且都属于一个新物种。它们的基因组大小为6.4 - 6.7 Mb，比其他可培养感染性簇1菌株的基因组小，但比通过ANI评估为其最接近邻居的不可培养感染性簇1 Sp+菌株AgTrS的基因组大。比较基因组分析确定了两个基因组中固氮所必需的基因、关于COG的基因组成、次生代谢物簇以及来自可培养感染性簇1菌株典型的转录调节因子。在其他可培养感染性菌株中存在的459个基因在这两个基因组中缺失，分布在整个基因组中，这表明这两个菌株正在发生基因组侵蚀。
