Draft Genomes of Symbiotic Strains AgB32 and AgKG'84/4 from Root Nodules of growing under Contrasted Environmental Conditions.

The genomes of two nitrogen-fixing strains, AgB32 and AgKG'84/4, were isolated from spore-containing (spore+) and spore-free (spore-) root nodules of , but they did not sporulate upon reinfection. The two strains are described as representatives of two novel candidate species Phylogenomic and ANI analyses indicate that each strain represents a novel species within cluster 1, with genome sizes of 6.3 and 6.7 Mb smaller than or similar to those of other cultivated -infective cluster 1 strains. Genes essential for nitrogen-fixation, clusters of orthologous genes, secondary metabolite clusters and transcriptional regulators analyzed by comparative genomic analyses were typical of those from -infective cluster 1 cultivated strains in both genomes. Compared to other cultivated -infective strains with large genomes, those of AgB32 and AgKG'84/4 had lost 380 or 409 genes, among which one cluster, one gene and the cluster, which indicates genome erosion is taking place in these two strains.