Emory Vaccine Center, Atlanta, GA, USA.
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA.
STAR Protoc. 2022 Jun 10;3(2):101391. doi: 10.1016/j.xpro.2022.101391. eCollection 2022 Jun 17.
We present a protocol to localize T cell receptor clones using the Visium spatial transcriptomics platform. This approach permits simultaneous localization of both gene expression and T cell clonotypes in situ within tissue sections. T cell receptor sequences identified by this protocol are readily recapitulated by single-cell sequencing. This technique enables detailed studies of the spatial organization of the human T cell repertoire, such as the localization of infiltrating T cell clones within the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Sudmeier et al. (2022).
我们提出了一种使用 Visium 空间转录组学平台定位 T 细胞受体克隆的方案。这种方法允许在组织切片的原位同时定位基因表达和 T 细胞克隆型。通过该方案鉴定的 T 细胞受体序列可以通过单细胞测序轻易地重现。该技术能够对人类 T 细胞库的空间组织进行详细研究，例如浸润性 T 细胞克隆在肿瘤微环境中的定位。有关该方案使用和执行的完整详细信息，请参阅 Sudmeier 等人（2022 年）。
