Thuwanut Paweena, Pimpin Alongkorn, Thatsanabunjong Fueangrat, Srisuwatanasagul Sayamon, Sereepapong Wisan, Sirayapiwat Porntip
Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Research Unit of Reproductive Medicine and Fertility Preservation, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Obstet Gynecol Sci. 2022 Jul;65(4):376-381. doi: 10.5468/ogs.22012. Epub 2022 Jun 16.
This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device, MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice were divided into three groups: 1) fresh control, 2) conventional culture system (control), and 3) non-conventional system with MHPD. Ovarian tissues were cultured for 96 hours and evaluated for follicle morphology, developmental stage, intact follicle density, and relative gene expression levels (proliferating cell nuclear antigen, insulin like growth factor 1, BAX, and Bcl-2). Our major data demonstrated that the mean percentage of primary follicle development was increased by the MHPD (P<0.05). In addition, this device could maintain and support follicle development better than the conventional culture systems. However, the overall outcomes were not significantly improved by our first-design prototype. Consequently, nextgeneration platforms should be developed as alternative medical tools for fertility preservation research.
本研究旨在使用小鼠模型比较传统和非传统（改良型静水微流无泵装置，MHPD）系统对卵巢组织培养和体外卵泡生长的效果。从7只野生型小鼠获取的总共56个卵巢皮质组织被分为三组：1）新鲜对照组，2）传统培养系统（对照组），3）带有MHPD的非传统系统。卵巢组织培养96小时，并对卵泡形态、发育阶段、完整卵泡密度和相对基因表达水平（增殖细胞核抗原、胰岛素样生长因子1、BAX和Bcl-2）进行评估。我们的主要数据表明，MHPD使初级卵泡发育的平均百分比增加（P<0.05）。此外，该装置比传统培养系统能更好地维持和支持卵泡发育。然而，我们的第一代设计原型并未显著改善总体结果。因此，应开发下一代平台作为生育力保存研究的替代医学工具。
