ICMR-National Institute of Malaria Research, New Delhi, India.
J Vector Borne Dis. 2022 Jan-Mar;59(1):29-36. doi: 10.4103/0972-9062.331409.
Diagnosis of malaria is a prominent challenge due to the endemic nature of infection. Malaria poses a great threat to global public health. The disease can be diagnosed by several techniques out of which microscopy is a known gold standard. High sensitivity of molecular techniques is making them more reliable and popular as tools for diagnosis of malaria. However, new methods are required which can fulfill the criteria of being Point of Care Test (POCT) as defined by WHO. Loop-mediated isothermal amplification (LAMP) technique amplifies DNA in an isothermal condition, and surpasses the disadvantages of conventional molecular techniques such as polymerase chain reaction. Multiplex LAMP, a modification of LAMP may emerge as a new POC for malaria diagnosis. This review deals with the use of LAMP and multiplex LAMP in diagnosis of malaria and its prospective use as point of care techniques.
疟疾的诊断是一个突出的挑战，因为感染具有地方性。疟疾对全球公共卫生构成了巨大威胁。这种疾病可以通过几种技术来诊断，其中显微镜检查是一种已知的金标准。分子技术的高灵敏度使它们作为疟疾诊断工具更加可靠和流行。然而，需要新的方法来满足世界卫生组织（WHO）定义的“即时检测（POCT）”标准。环介导等温扩增（LAMP）技术在等温条件下扩增 DNA，并克服了聚合酶链反应等传统分子技术的缺点。多重 LAMP 是 LAMP 的一种改进，可能成为疟疾诊断的新即时检测方法。这篇综述讨论了 LAMP 和多重 LAMP 在疟疾诊断中的应用及其作为即时检测技术的潜在用途。
