CEITEC MU-Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Faculty of Science, National Centre for Biomolecular Research, Masaryk University, Brno, Czech Republic.
Plant Cell. 2022 Aug 25;34(9):3200-3213. doi: 10.1093/plcell/koac179.
The phytohormone auxin is a master regulator of plant growth and development in response to many endogenous and environmental signals. The underlying coordination of growth is mediated by the formation of auxin maxima and concentration gradients. The visualization of auxin dynamics and distribution can therefore provide essential information to increase our understanding of the mechanisms by which auxin orchestrates these growth and developmental processes. Several auxin reporters have been developed to better perceive the auxin distribution and signaling machinery in vivo. This review focuses on different types of auxin reporters and biosensors used to monitor auxin distribution and its dynamics, as well as auxin signaling, at the cellular and tissue levels in different plant species. We provide a brief history of each reporter and biosensor group and explain their principles and utilities.
植物激素生长素是植物生长和发育的主要调节剂，它可以响应许多内源性和环境信号。生长的协调是通过生长素最大值和浓度梯度的形成来介导的。因此，生长素动态和分布的可视化可以提供重要信息，以增加我们对生长素协调这些生长和发育过程的机制的理解。已经开发了几种生长素报告基因来更好地感知体内的生长素分布和信号机制。本综述重点介绍了不同类型的生长素报告基因和生物传感器，用于监测不同植物物种中细胞和组织水平的生长素分布及其动态，以及生长素信号。我们简要介绍了每个报告基因和生物传感器组的历史，并解释了它们的原理和用途。
