Department of Biosciences, Durham University, South Road, Durham DH1 3LE, UK; Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Department of Biosciences, Durham University, South Road, Durham DH1 3LE, UK.
Plant Commun. 2024 Jul 8;5(7):100886. doi: 10.1016/j.xplc.2024.100886. Epub 2024 Mar 19.
The interaction between auxin and cytokinin is important in many aspects of plant development. Experimental measurements of both auxin and cytokinin concentration and reporter gene expression clearly show the coexistence of auxin and cytokinin concentration patterning in Arabidopsis root development. However, in the context of crosstalk among auxin, cytokinin, and ethylene, little is known about how auxin and cytokinin concentration patterns simultaneously emerge and how they regulate each other in the Arabidopsis root. This work utilizes a wide range of experimental observations to propose a mechanism for simultaneous patterning of auxin and cytokinin concentrations. In addition to revealing the regulatory relationships between auxin and cytokinin, this mechanism shows that ethylene signaling is an important factor in achieving simultaneous auxin and cytokinin patterning, while also predicting other experimental observations. Combining the mechanism with a realistic in silico root model reproduces experimental observations of both auxin and cytokinin patterning. Predictions made by the mechanism can be compared with a variety of experimental observations, including those obtained by our group and other independent experiments reported by other groups. Examples of these predictions include patterning of auxin biosynthesis rate, changes in PIN1 and PIN2 patterns in pin3,4,7 mutants, changes in cytokinin patterning in the pls mutant, PLS patterning, and various trends in different mutants. This research reveals a plausible mechanism for simultaneous patterning of auxin and cytokinin concentrations in Arabidopsis root development and suggests a key role for ethylene pattern integration.
生长素和细胞分裂素的相互作用在植物发育的许多方面都很重要。生长素和细胞分裂素浓度的实验测量以及报告基因表达的实验测量清楚地表明，生长素和细胞分裂素浓度模式在拟南芥根发育中同时存在。然而，在生长素、细胞分裂素和乙烯之间的串扰背景下，人们对生长素和细胞分裂素浓度模式如何同时出现以及它们如何相互调节知之甚少。这项工作利用广泛的实验观察结果，提出了生长素和细胞分裂素浓度同时模式化的机制。该机制不仅揭示了生长素和细胞分裂素之间的调控关系，还表明乙烯信号是实现生长素和细胞分裂素同时模式化的重要因素，同时还预测了其他实验观察结果。将该机制与现实的根模型相结合，可以再现生长素和细胞分裂素模式化的实验观察结果。该机制的预测可以与各种实验观察结果进行比较，包括我们小组和其他独立小组报告的其他实验观察结果。这些预测的例子包括生长素生物合成速率的模式化、pin3,4,7 突变体中 PIN1 和 PIN2 模式的变化、pls 突变体中细胞分裂素模式的变化、PLS 模式化以及不同突变体中的各种趋势。这项研究揭示了生长素和细胞分裂素在拟南芥根发育中同时模式化的合理机制，并提出了乙烯模式整合的关键作用。
