Department of Operative Dentistry and Periodontology, University Medical Center Rostock, Rostock, Germany.
Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Rostock, Rostock, Germany.
Clin Implant Dent Relat Res. 2022 Oct;24(5):664-675. doi: 10.1111/cid.13114. Epub 2022 Jun 16.
Inflammation triggered by bacterial biofilms in the surrounding tissue is a major etiological factor for peri-implantitis and subsequent implant failure. However, little is known about the direct effects of bacterial corrosion and recolonization on implant failure PURPOSE: To investigate the influence of oral commensals on bacterial corrosion and recolonization of titanium surfaces.
Streptococcus sanguinis (S. sanguinis) and Porphyromonas gingivalis (P. gingivalis), which are key bacteria in oral biofilm formation, were cultured on commercially pure titanium and titanium-aluminum-vanadium (Ti6Al4V) plates in artificial saliva/brain heart infusion medium under aerobic or anaerobic conditions. Biofilm formation was examined after 7 and 21 days by crystal violet and live/dead staining. Titanium ions released into culture supernatants were analyzed over a period of 21 days by atomic absorption spectrometry. Visual changes in surface morphology were investigated using scanning electron microscopy. Biofilm formation on sterilized, biocorroded, and recolonized implant surfaces was determined by crystal violet staining.
S. sanguinis and P. gingivalis formed stable biofilms on the titanium samples. Bacterial corrosion led to a significant increase in titanium ion release from these titanium plates (p < 0.01), which was significantly higher under aerobic conditions on pure titanium (p ≤ 0.001). No obvious morphological surface changes, such as pitting and discoloration, were detected in the titanium samples. During early biofilm formation, the addition of titanium ions significantly decreased the number of live cells. In contrast, a significant effect on biofilm mass was only detected with P. gingivalis. Bacterial corrosion had no influence on bacterial recolonization following sterilization of titanium and Ti6Al4V surfaces.
Bacterial corrosion differs between oral commensal bacteria and leads to increased titanium ion release from titanium plates. The titanium ion release did not influence biofilm formation or bacterial recolonization under in vitro conditions.
周围组织中的细菌生物膜引发的炎症是导致种植体周围炎和随后种植体失败的主要病因。然而，对于细菌腐蚀和再定植对种植体失败的直接影响知之甚少。目的：研究口腔共生菌对钛表面细菌腐蚀和再定植的影响。
将口腔生物膜形成的关键细菌血链球菌（S. sanguinis）和牙龈卟啉单胞菌（P. gingivalis）在人工唾液/脑心浸液培养基中，于有氧或无氧条件下在商用纯钛和钛铝钒（Ti6Al4V）板上培养。在第 7 天和第 21 天通过结晶紫和死活染色检查生物膜形成情况。通过原子吸收光谱法在 21 天内分析释放到培养上清液中的钛离子。通过扫描电子显微镜观察表面形貌的变化。通过结晶紫染色确定灭菌、生物腐蚀和再定植后种植体表面生物膜的形成。
S. sanguinis 和 P. gingivalis 在钛样品上形成了稳定的生物膜。细菌腐蚀导致这些钛板中钛离子的释放显著增加（p<0.01），在纯钛上有氧条件下增加更为明显（p≤0.001）。钛样品未检测到明显的表面形貌变化，如点蚀和变色。在早期生物膜形成过程中，添加钛离子显著减少了活细胞数量。相比之下，仅在 P. gingivalis 中检测到对生物膜质量的显著影响。细菌腐蚀对钛和 Ti6Al4V 表面灭菌后的细菌再定植没有影响。
口腔共生菌之间的细菌腐蚀不同，导致钛板中钛离子的释放增加。在体外条件下，钛离子释放不会影响生物膜形成或细菌再定植。
