Wang Dan, Yue Yingying, Liu He, Zhang Tian, Haney Evan F, Hancock Robert E W, Yu Jian, Shen Ya
Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Division of Endodontics, Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, BC, Canada.
Front Bioeng Biotechnol. 2024 Jan 10;11:1339912. doi: 10.3389/fbioe.2023.1339912. eCollection 2023.
Titanium alloys have gained popularity in implant dentistry for the restoration of missing teeth and related hard tissues because of their biocompatibility and enhanced strength. However, titanium corrosion and infection caused by microbial biofilms remains a significant clinical challenge leading to implant failure. This study aimed to evaluate the effectiveness of antibiofilm peptides 1018 and DJK-5 on the corrosion resistance of titanium in the presence of . Commercially pure titanium disks were prepared and used to form biofilms. The disks were randomly assigned to different treatment groups (exposed to supplied with sucrose) including a positive control with untreated biofilms, peptides 1018 or DJK-5 at concentrations of 5 μg/mL or 10 μg/mL, and a negative control with no . Dynamic biofilm growth and pH variation of all disks were measured after one or two treatment periods of 48 h. After incubation, the dead bacterial proportion, surface morphology, and electrochemical behaviors of the disks were determined. The results showed that peptides 1018 and DJK-5 exhibited significantly higher dead bacterial proportions than the positive control group in a concentration dependent manner ( < 0.01), as well as far less defects in microstructure. DJK-5 at 10 μg/mL killed 84.82% of biofilms and inhibited biofilm growth, preventing acidification due to and maintaining a neutral pH. Potential polarization and electrochemical impedance spectroscopy data revealed that both peptides significantly reduced the corrosion and passive currents on titanium compared to titanium surfaces with untreated biofilms, and increased the resistance of the passive film ( < 0.05), with 10 μg/mL of DJK-5 achieving the greatest effect. These findings demonstrated that antibiofilm peptides are effective in promoting corrosion resistance of titanium against , suggesting a promising strategy to enhance the stability of dental implants by endowing them with antibiofilm and anticorrosion properties.
由于钛合金具有生物相容性和更高的强度，因此在种植牙科中用于修复缺失牙齿及相关硬组织方面颇受青睐。然而，钛的腐蚀以及微生物生物膜引起的感染仍是导致种植失败的重大临床挑战。本研究旨在评估抗生物膜肽1018和DJK - 5在[具体物质未提及]存在的情况下对钛耐腐蚀性的影响。制备了商业纯钛盘并用于形成生物膜。将这些盘随机分配到不同的处理组（暴露于添加蔗糖的[具体物质未提及]中），包括未处理生物膜的阳性对照组、浓度为5μg/mL或10μg/mL的肽1018或DJK - 5处理组，以及无[具体物质未提及]的阴性对照组。在48小时的一个或两个处理周期后，测量所有盘的动态生物膜生长和pH变化。孵育后，测定盘的死菌比例、表面形态和电化学行为。结果表明，肽1018和DJK - 5以浓度依赖性方式显示出比阳性对照组显著更高的死菌比例（P < 0.01），并且微观结构缺陷也少得多。10μg/mL的DJK - 5杀死了84.82%的生物膜并抑制了生物膜生长，防止了因[具体物质未提及]导致的酸化并维持中性pH。动电位极化和电化学阻抗谱数据表明，与未处理生物膜的钛表面相比，两种肽均显著降低了钛上的腐蚀电流和钝化电流，并增加了钝化膜的电阻（P < 0.05），10μg/mL的DJK - 5效果最佳。这些发现表明，抗生物膜肽在促进钛对[具体物质未提及]的耐腐蚀性方面是有效的，这表明通过赋予牙科植入物抗生物膜和抗腐蚀特性来提高其稳定性是一种有前景的策略。
