Department of Chemistry, University of Reading, Whiteknights, Reading, UK.
Proteomics. 2022 Aug;22(15-16):e2100394. doi: 10.1002/pmic.202100394. Epub 2022 Jun 26.
Omics analysis by mass spectrometry (MS) is a vast field, with proteomics, metabolomics and lipidomics dominating recent research by exploiting biological MS ionisation techniques. Traditional MS ionisation techniques such as electrospray ionisation have limitations in analyte-specific sensitivity, modes of sampling and throughput, leading to many researchers investigating new ionisation methods for omics research. In this review, we examine the current landscape of these new ionisation techniques, divided into the three groups of (electro)spray-based, laser-based and other miscellaneous ionisation techniques. Due to the wide range of new developments, this review can only provide a starting point for further reading on each ionisation technique, as each have unique benefits, often for specialised applications, which promise beneficial results for different areas in the omics world.
质谱（MS）的组学分析是一个广阔的领域，蛋白质组学、代谢组学和脂质组学利用生物 MS 离子化技术主导着最近的研究。传统的 MS 离子化技术，如电喷雾离子化，在分析物特异性灵敏度、采样方式和通量方面存在局限性，这导致许多研究人员正在研究新的用于组学研究的离子化方法。在这篇综述中，我们检查了这些新的离子化技术的现状，将其分为基于（电）喷雾的、基于激光的和其他各种离子化技术三组。由于新的发展范围广泛，因此本综述只能为每种离子化技术的进一步阅读提供一个起点，因为每种技术都有独特的优势，通常适用于特定的应用，这有望为组学领域的不同领域带来有益的结果。
