Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, China.
Department of Chemistry, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Anal Chem. 2022 Jun 28;94(25):9176-9183. doi: 10.1021/acs.analchem.2c01868. Epub 2022 Jun 16.
In this work, based on electrochemiluminescence (ECL) technology and self-assembled portable disease detection chips, a bioactivity-maintained sensing platform was developed for the quantitative detection of neuron-specific enolase. First, we prepared FeO@MoS nanocomposites as an efficient catalyst to accelerate the reduction of persulfate (SO). Specifically, abundant sulfate radicals (SO) were generated because of cyclic conversion between Fe and Fe. Meanwhile, MoS nanoflowers with a high specific surface area could not only load more FeO but also solve its agglomeration problem, which greatly improved the catalytic efficiency. Moreover, a biosensor chip was constructed by standard lithography processes for disease detection, which had good sensitivity and portability. According to the above strategies, the developed portable sensing platform played the part of promoting the practical application of bioanalysis in early tumor screening and clinical diagnosis. In addition, we developed a short peptide ligand (NARKFYKG, NAR) to avoid the occupation of antigen binding sites by specifically connecting to Fc fragments in antibodies. Thus, the binding efficiency of antibodies and the activity of biosensors were improved due to the introduction of NAR.
在这项工作中，我们基于电致化学发光（ECL）技术和自组装便携式疾病检测芯片，开发了一种保持生物活性的传感平台，用于定量检测神经元特异性烯醇化酶。首先，我们制备了 FeO@MoS 纳米复合材料作为高效催化剂，以加速过硫酸盐（SO）的还原。具体来说，由于 Fe 和 Fe 之间的循环转化，大量的硫酸根自由基（SO）生成。同时，具有高比表面积的 MoS 纳米花不仅可以负载更多的 FeO，还可以解决其团聚问题，从而大大提高了催化效率。此外，通过标准光刻工艺构建了用于疾病检测的生物传感器芯片，该芯片具有良好的灵敏度和便携性。根据上述策略，开发的便携式传感平台在促进生物分析在早期肿瘤筛查和临床诊断中的实际应用方面发挥了作用。此外，我们开发了一种短肽配体（NARKFYKG，NAR），通过特异性连接抗体中的 Fc 片段，避免抗原结合位点的占据。因此，由于 NAR 的引入，提高了抗体的结合效率和生物传感器的活性。
