Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, Shandong, China.
ACS Sens. 2022 Aug 26;7(8):2273-2280. doi: 10.1021/acssensors.2c00819. Epub 2022 Aug 2.
Herein, we reported an efficient electrochemiluminescence (ECL) biosensor chip for sensitive detection of neuron-specific enolase (NSE). First, 3,4,9,10-perylenetetracarboxylic acid with good luminescence characteristics was used as a luminophore to obtain a stable ECL signal. Subsequently, hollow porous CoO/CuO concave polyhedron nanocages (CPNCs) were designed as co-reaction promoters to amplify the luminescence signals for highly sensitive trace detection of NSE. In brief, the rapid cyclic conversion of Co/Co and Cu/Cu redox pairs could continuously catalyze the reduction of persulfate (SO), thus providing a large number of essential active intermediates (SO) for ECL emission. Meanwhile, the unique structure of CoO/CuO CPNCs possessed a large specific surface area, which greatly improved its catalytic efficiency. Third, NKFRGKYKC was developed as an affinity ligand for specific antibody fixation, which improved incubation efficiency and protected bioactivity of antibodies. Finally, we independently designed a microchip and applied it for ECL detection to improve the practical application ability of the sensor. The developed biosensor exhibited good sensitivity with a wide linear range (10 fg/mL to 100 ng/mL) and a low detection limit (3.42 fg/mL), which played an active role in the clinical application of sensing analysis.
本文报道了一种用于灵敏检测神经元特异性烯醇化酶（NSE）的高效电化学发光（ECL）生物传感器芯片。首先，我们使用具有良好发光特性的 3,4,9,10-苝四羧酸作为发光体，以获得稳定的 ECL 信号。随后，设计了中空多孔 CoO/CuO 凹多面体纳米笼（CPNCs）作为共反应促进剂，以放大发光信号，用于高度灵敏的痕量 NSE 检测。简而言之，Co/Co 和 Cu/Cu 氧化还原对的快速循环转换可以不断催化过硫酸盐（SO₄²⁻）的还原，从而为 ECL 发射提供大量必需的活性中间体（SO₄²⁻）。同时，CoO/CuO CPNCs 的独特结构具有较大的比表面积，大大提高了其催化效率。第三，NKFRGKYKC 被开发为用于特定抗体固定的亲和配体，提高了孵育效率并保护了抗体的生物活性。最后，我们独立设计了一个微芯片并将其应用于 ECL 检测，以提高传感器的实际应用能力。所开发的生物传感器具有良好的灵敏度，线性范围宽（10 fg/mL 至 100 ng/mL），检测限低（3.42 fg/mL），在传感分析的临床应用中发挥了积极作用。
