Department of Parasitology and Invasive Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
J Eukaryot Microbiol. 2023 Jan;70(1):e12931. doi: 10.1111/jeu.12931. Epub 2022 Jun 29.
Honey bees are among the most effective pollinators that promote plant reproduction. Bees are highly active in the pollen collection season, which can lead to the transmission of selected pathogens between colonies. The clade Starmerella comprises yeasts that are isolated mainly from bees and their environment. When visiting plants, bees can come into contact with Starmerella spp. The aim of this study was to determine the prevalence and phylogenetic position of S. apis in bee colonies. Bee colonies were collected from nine apiaries in three regions. Ten colonies were sampled randomly from each apiary, and pooled samples were collected from the central part of the hive in each colony. A total of 90 (100%) bee colonies from nine apiaries were examined. Starmerella apis was detected in 31 (34.44%) samples, but related species were not identified. The 18S rRNA amplicon sequences of S. apis were compatible with the GenBank sequences of Starmerella spp. from India, Japan, Syria, Thailand, and the USA. The amplicon sequences of S. apis were also 99.06% homologous with the sequences deposited in GenBank under accession numbers JX515988 and NG067631.This is the first study to perform a phylogenetic analysis of S. apis in Polish honey bees.
蜜蜂是促进植物繁殖的最有效传粉者之一。在花粉采集季节，蜜蜂非常活跃，这可能导致种群之间传播选定的病原体。Starmerella 菌属是主要从蜜蜂及其环境中分离出来的酵母。当蜜蜂访问植物时，它们可能会接触到 Starmerella 属。本研究旨在确定蜜蜂种群中 S.apis 的流行程度和系统发育位置。从三个地区的九个养蜂场收集了蜜蜂种群。从每个养蜂场随机抽取 10 个蜂群进行采样，从每个蜂群的蜂巢中央采集混合样本。共检查了来自九个养蜂场的 90 个（100%）蜜蜂种群。在 31 个（34.44%）样本中检测到 S.apis，但未鉴定出相关物种。S.apis 的 18S rRNA 扩增子序列与来自印度、日本、叙利亚、泰国和美国的 Starmerella 属的 GenBank 序列兼容。S.apis 的扩增子序列与在 GenBank 中以 JX515988 和 NG067631 登记号储存的序列也有 99.06%的同源性。这是首次在波兰蜜蜂中对 S.apis 进行系统发育分析的研究。
