Yu Xiaojuan, Fu Xiaoxue, Yang Jiaxin, Chen Lu, Leng Feng, Yang Zhangyou, Yu Chao
Chongqing Key Laboratory for Pharmaceutical Metabolism Research, Chongqing Pharmacodynamic Evaluation Engineering Technology Research Center, College of Pharmacy, Chongqing Medical University, Chongqing, 400016, PR China.
Mater Today Bio. 2022 Jun 2;15:100308. doi: 10.1016/j.mtbio.2022.100308. eCollection 2022 Jun.
Diabetic wounds have an extremely complex microenvironment of hyperglycemia, hypoxia and high reactive oxygen species (ROS). Therefore, the regulation and management of this microenvironment may provide a new and improved treatment method for chronic diabetic wound healing. Herein, a glucose/ROS cascade-responsive nanozyme (CHA@GOx) was developed for diabetic wound treatment based on Ce-driven coassembly by a special dual ligand (alendronic acid and 2-methylimidazole) and glucose oxidase (GOx). It possesses superoxide dismutase and catalase mimic activities, which effectively remove excess ROS. In particular, it can catalyze excessive hydrogen peroxide generated by the glucose oxidation reaction to produce oxygen, regulate the oxygen balance of the wound, and reduce the toxic side effects of GOx, thus achieving the purpose of synergistically repairing diabetic wounds. experiments show that CHA@GOx assists mouse fibroblast migration and promotes human umbilical vein endothelial cell tube formation. , it can induce angiogenesis, collagen deposition, and re-epithelialization during wound healing in diabetic mice. Taken together, this study indicates that the coassembly of multifunctional nanozymes has implications in diabetic wound healing.
糖尿病伤口具有高血糖、缺氧和高活性氧（ROS）的极其复杂的微环境。因此，对这种微环境的调控和管理可能为慢性糖尿病伤口愈合提供一种新的、改良的治疗方法。在此，基于铈驱动的共组装，通过特殊的双配体（阿仑膦酸和2-甲基咪唑）和葡萄糖氧化酶（GOx）开发了一种葡萄糖/ROS级联响应纳米酶（CHA@GOx）用于糖尿病伤口治疗。它具有超氧化物歧化酶和过氧化氢酶模拟活性，能有效清除过量的ROS。特别地，它可以催化葡萄糖氧化反应产生的过量过氧化氢生成氧气，调节伤口的氧平衡，并降低GOx的毒副作用，从而实现协同修复糖尿病伤口的目的。实验表明，CHA@GOx有助于小鼠成纤维细胞迁移并促进人脐静脉内皮细胞形成管腔。此外，它可以在糖尿病小鼠伤口愈合过程中诱导血管生成、胶原蛋白沉积和再上皮化。综上所述，本研究表明多功能纳米酶的共组装在糖尿病伤口愈合中具有重要意义。
