精子冷冻保存与 DNA 甲基化：对辅助生殖技术成功和后代健康的潜在影响。
Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring.
机构信息
Clinical Research Development Unit, Amiralmomenin Hospital, Arak University of Medical Sciences, Arak, Iran.
Department of Urology, University of Miami, Miller School of Medicine, Miami, FL, USA.
出版信息
J Assist Reprod Genet. 2022 Aug;39(8):1815-1824. doi: 10.1007/s10815-022-02545-6. Epub 2022 Jun 17.
Abstract
Despite the beneficial effects of sperm cryopreservation, increased reactive oxygen species (ROS) production during this process can affect spermatozoon structure and function. Moreover, ROS production is associated with elevated DNA damage and alterations in DNA methylation. There is little information about the effects of cryopreservation on epigenetic modulation in sperm and the health of children born with frozen spermatozoa. Considering the potential consequences of cryopreservation in ART-conceived children, it is necessary to assure that cryopreservation does not modify sperm DNA methylation status. This review summarizes reports on epigenetic modifications of spermatozoa during cryopreservation and the probable effects of this process on offspring health. Contradictory results have reported the influence of sperm cryopreservation on DNA methylation in imprinted genes. Multiclinical studies with larger sample sizes under the same conditions of cryopreservation and DNA methylation analysis are needed to make any definitive conclusion about the effect of the cryopreservation process on sperm DNA methylation.
摘要
尽管精子冷冻保存有有益的效果，但是在这个过程中活性氧（ROS）的产生会影响精子的结构和功能。此外，ROS 的产生与 DNA 损伤的增加和 DNA 甲基化的改变有关。关于冷冻保存对精子表观遗传调节和冷冻精子生育的儿童健康的影响，信息很少。考虑到冷冻保存对 ART 受孕儿童的潜在影响，有必要确保冷冻保存不会改变精子 DNA 甲基化状态。这篇综述总结了关于冷冻保存过程中精子表观遗传修饰的报告，以及这一过程对后代健康的可能影响。关于精子冷冻保存对印记基因 DNA 甲基化的影响，有报道称结果存在矛盾。需要在相同的冷冻保存和 DNA 甲基化分析条件下进行更大规模的多临床研究，才能对冷冻保存过程对精子 DNA 甲基化的影响得出任何明确的结论。
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