冷冻保存的人类精子的储存时间不影响与生育相关的途径。
The storage time of cryopreserved human spermatozoa does not affect pathways involved in fertility.
作者信息
Stigliani Sara, Amaro Adriana, Reggiani Francesco, Maccarini Elena, Massarotti Claudia, Lambertini Matteo, Anserini Paola, Scaruffi Paola
机构信息
SS Physiopathology of Human Reproduction, IRCCS Ospedale Policlinico San Martino, Genova, Italy.
SSD Regolazione dell'Espressione Genica, IRCCS Ospedale Policlinico San Martino, Genova, Italy.
出版信息
Basic Clin Androl. 2024 Sep 17;34(1):15. doi: 10.1186/s12610-024-00231-4.
BACKGROUND
Cryopreservation of human spermatozoa is a widely used technique in the assisted reproduction technology laboratory for the storage of gametes for later use, for the fertility preservation and for sperm donation programs. Cryopreservation can cause damage to membrane, cytoskeletal, acrosome and increased oxidative stress, sperm DNA damage and transcriptome changes. To assess the impact of storage time on the transcriptome of frozen human spermatozoa, semen samples were collected from 24 normospermic donors of whom 13 had cryostored semen for a short-time (1 week) and 11 had cryostored semen for a long-time (median 9 years).
RESULTS
RNA was extracted from each frozen-thawed sperm sample, randomized in pools, and analyzed by microarrays. Five transcripts were in higher abundance in the long-time respect to the short-time storage group. Functional annotation enrichment disclosed that that the length of cryostorage has no effect on critical pathways involved in sperm physiology and function.
CONCLUSIONS
The storage time of cryopreserved human spermatozoa does not affect pathways involved in fertility.
背景
人类精子冷冻保存是辅助生殖技术实验室中广泛应用的一项技术，用于储存配子以供日后使用、生育力保存以及精子捐赠项目。冷冻保存可导致精子膜、细胞骨架、顶体受损，并增加氧化应激、精子DNA损伤以及转录组变化。为评估储存时间对冷冻人类精子转录组的影响，从24名正常精子捐献者中采集精液样本，其中13人短期（1周）冷冻保存精液，11人长期（中位数9年）冷冻保存精液。
结果
从每个冻融精子样本中提取RNA，随机分组，通过微阵列进行分析。与短期储存组相比，长期储存组中有5种转录本丰度更高。功能注释富集分析表明，冷冻保存时间对精子生理和功能所涉及的关键通路没有影响。
结论
冷冻保存的人类精子的储存时间不影响生育相关通路。
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