Swanson S P, Nicoletti J, Rood H D, Buck W B, Cote L M, Yoshizawa T
J Chromatogr. 1987 Mar 6;414(2):335-42. doi: 10.1016/0378-4347(87)80058-0.
The three trichothecene mycotoxins T-2 toxin, diacetoxyscirpenol (DAS) and deoxynivalenol (DON) were incubated in vitro for 12, 24 and 48 h with rumen microorganisms obtained from a fistulated dairy cow. Gas chromatographic and gas chromatographic-mass spectrometric analyses of extracts indicated all three toxins were biotransformed to a variety of deepoxy and deacylated products. DON was partially converted to a product identified as deepoxy DON. DAS was rapidly converted to four products including 15-monoacetoxyscirpenol (MAS), scirpentriol and two new compounds identified as 15-acetoxy-3 alpha,4 beta-dihydroxytrichothec-9,12-diene (deepoxy MAS) and 3 alpha,4 beta,15-trihydroxytrichothec-9,12-diene (deepoxy scirpentriol). T-2 toxin was also completely biotransformed to the products HT-2, T-2 triol and two new metabolites identified as 15-acetoxy-3 alpha,4 beta-dihydroxy-8 alpha-(3-methylbutyryloxy) trichothec-9,12-diene (deepoxy HT-2) and 3 alpha,4 beta,15-trihydroxy-8 alpha-(3-methylbutyryloxy)trichothec-9,12-diene (deepoxy T-2 triol).
将三种单端孢霉烯族霉菌毒素T-2毒素、二醋酸藨草镰刀菌烯醇（DAS）和脱氧雪腐镰刀菌烯醇（DON），与从一头装有瘤胃瘘管的奶牛获取的瘤胃微生物在体外培养12、24和48小时。提取物的气相色谱和气相色谱-质谱分析表明，所有三种毒素均被生物转化为多种脱环氧和脱酰基产物。DON部分转化为一种鉴定为脱环氧DON的产物。DAS迅速转化为四种产物，包括15-单醋酸藨草镰刀菌烯醇（MAS）、蛇形菌素以及两种新化合物，分别鉴定为15-乙酰氧基-3α,4β-二羟基单端孢霉-9,12-二烯（脱环氧MAS）和3α,4β,15-三羟基单端孢霉-9,12-二烯（脱环氧蛇形菌素）。T-2毒素也完全生物转化为HT-2、T-2三醇以及两种新的代谢产物，分别鉴定为15-乙酰氧基-3α,4β-二羟基-8α-（3-甲基丁酰氧基）单端孢霉-9,12-二烯（脱环氧HT-2）和3α,4β,15-三羟基-8α-（3-甲基丁酰氧基）单端孢霉-9,12-二烯（脱环氧T-2三醇）。
