National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
National Aquafeed Safety Assessment Center, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2022 Sep;43:101007. doi: 10.1016/j.cbd.2022.101007. Epub 2022 Jun 8.
Largemouth bass is sensitive to the dietary starch level and excess starch can induce metabolic liver diseases (MLD). Hepatic fibrosis is a typical pathological phenotype of MLD in largemouth bass, but the molecular basis underlying is largely unclear. This study fed fish with a low or high starch diet for 4 weeks. Liver tissues with or without fibrotic symptoms were recognized through histopathological and molecular markers analysis of hepatic fibrosis, following TMT Quantitative proteomics and conducted Parallel Reaction Monitoring (PRM) analyses. 2455 differentially expressed proteins with 1618 up-regulated and 837 down-regulated were identified in this study. In GO terms, up-regulated proteins were correlated with cytoskeleton organization, supramolecular fiber, cytoskeleton protein binding, and actin-binding, while down-regulated proteins were involved in mainly metabolism-related processes, and molecular binding activity. Down-regulated proteins were enriched in 63 KEGG pathways and concentrated in metabolism-related pathways, especially glucose, lipid, and amino acid metabolism. 70 KEGG pathways of up-regulated proteins mainly included immunity and inflammation-related pathways. The expression trends of 11 differentially expressed proteins were consistent with proteome results by PRM analysis. In conclusion, the development of hepatic fibrosis induced by high starch may be related to multi-signaling pathways, metabolism processes, and targets, which provides important data for further study on revealing the molecular mechanism of hepatic fibrosis.
大口黑鲈对膳食淀粉水平敏感，过量的淀粉会诱导代谢性肝病（MLD）。肝纤维化是大口黑鲈 MLD 的典型病理表型，但其中的分子基础在很大程度上尚不清楚。本研究用低淀粉或高淀粉饲料喂养鱼 4 周。通过肝纤维化的组织病理学和分子标志物分析，识别出有或无纤维化症状的肝组织，随后进行 TMT 定量蛋白质组学和并行反应监测（PRM）分析。在这项研究中，共鉴定到 2455 个差异表达蛋白，其中 1618 个上调，837 个下调。在 GO 术语中，上调蛋白与细胞骨架组织、超分子纤维、细胞骨架蛋白结合和肌动蛋白结合相关，而下调蛋白主要与代谢相关过程和分子结合活性相关。下调蛋白富集在 63 个 KEGG 通路中，主要集中在代谢相关通路，特别是葡萄糖、脂质和氨基酸代谢。70 个上调蛋白的 KEGG 通路主要包括免疫和炎症相关通路。11 个差异表达蛋白的表达趋势与 PRM 分析的蛋白质组结果一致。总之，高淀粉诱导的肝纤维化的发展可能与多信号通路、代谢过程和靶点有关，为进一步研究肝纤维化的分子机制提供了重要数据。
