Brown D W, Beards G M, Flewett T H
J Clin Microbiol. 1987 Apr;25(4):637-40. doi: 10.1128/jcm.25.4.637-640.1987.
Enzyme immunoassays were developed for the detection of Breda virus antibody and antigen. Cattle sera collected in the United Kingdom were found to have a high prevalence of antibody (55%) to Breda virus when examined in a competitive enzyme-linked immunosorbent assay. A low prevalence of antibody was found in pigs (2.2%), and no antibody was found in sheep or goat sera. No antibody to either Breda virus or Berne virus was detected in human sera collected from veterinarians and farm workers. Only 1 of 430 human fecal specimens (0.2%) contained Breda virus antigen detectable by enzyme-linked immunosorbent assay.
已开发出酶免疫测定法用于检测布雷达病毒抗体和抗原。在英国采集的牛血清，通过竞争性酶联免疫吸附测定法检测时，发现对布雷达病毒抗体的流行率很高（55%）。在猪中发现抗体流行率较低（2.2%），而在绵羊或山羊血清中未发现抗体。从兽医和农场工人采集的人血清中未检测到针对布雷达病毒或伯尔尼病毒的抗体。在430份人类粪便标本中，只有1份（0.2%）含有可通过酶联免疫吸附测定法检测到的布雷达病毒抗原。
