Weiss M, Steck F, Kaderli R, Horzinek M C
Vet Microbiol. 1984 Oct;9(6):523-31. doi: 10.1016/0378-1135(84)90014-2.
After inoculation into 2 foals, Berne virus induced neutralizing antibody, but did not cause clinical symptoms. In a horizontal study of seropositive mares and their offspring, a decline of maternal antibodies and a sudden synchronous seroconversion in all foals were observed, again without clinical symptoms. The virus is widespread in the Swiss horse population and has been so during the last decade; rises in antibody titers were noted in 9% of paired sera sampled at random. Positive reactions were also obtained in serum neutralization tests and ELISA using small numbers of horse sera from Germany, France and the U.S.A. The results of neutralization tests and ELISA were correlated in 83% of random samples tested; 13% were neutralization-positive and ELISA-negative and in 4% the inverse was observed. Neutralizing activity was found in the sera of other ungulates (cattle, goat, sheep and pig), laboratory rabbits and 2 species of wild mice (Clethrionomys glareolus and Apodemus sylvaticus). Inconclusive results were obtained with feline and human sera; those from dogs and foxes (Vulpes vulpes) were consistently negative. The probable occurrence of antigenic variants in Berne-type viruses is discussed.
将伯尔尼病毒接种到2匹幼驹体内后，该病毒诱导产生了中和抗体，但未引发临床症状。在一项针对血清学阳性母马及其后代的横向研究中，观察到母源抗体下降，且所有幼驹均突然同步发生血清学转换，同样未出现临床症状。该病毒在瑞士马群中广泛传播，在过去十年一直如此；随机抽取的配对血清中，9%的血清抗体滴度有所上升。使用来自德国、法国和美国的少量马血清进行血清中和试验和酶联免疫吸附测定（ELISA），也得到了阳性反应。在83%的随机检测样本中，中和试验和ELISA的结果具有相关性；13%的样本中和试验呈阳性而ELISA呈阴性，4%的样本情况则相反。在其他有蹄类动物（牛、山羊、绵羊和猪）、实验用兔以及两种野生小鼠（大林姬鼠和黄胸鼠）的血清中发现了中和活性。猫和人的血清检测结果不明确；狗和狐狸（赤狐）的血清检测结果始终为阴性。文中还讨论了伯尔尼型病毒中可能存在的抗原变异体情况。
