China Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China.
Technology Center of Yunnan China Tobacco Industry Company, Kunming 650000, China.
Gene. 2022 Aug 15;835:146652. doi: 10.1016/j.gene.2022.146652. Epub 2022 Jun 14.
Sugars are both nutrients and important signal molecules in higher plants. Sugar transporters (STs) are involved in sugar loading and unloading and facilitate sugar transport across membranes. Tobacco (Nicotiana tabacum) is a model plant and one of the most significant plants economically. In our research, 92 N. tabacum ST (NtST) genes were identified and classified into eight distinct subfamilies in the tobacco genome based on phylogenetic analysis. Exon-intron analysis revealed that each subfamily manifested closely associated gene architectural features based on a comparable number or length of exons. Tandem repetition and purifying selection were the main factors of NtST gene evolution. A search for cis-regulatory elements in the promoter sequences of the NtST gene families suggested that they are probably regulated by light, plant hormones, and abiotic stress factors. We performed a comprehensive expression study in different tissues, viarious abiotic and phytohormone stresses. The results revealed different expression patterns and the functional diversification of NtST genes. The resulting data showed that NtSFP1 was highly expressed all measured five tobacco tissues, and also regulated by the MeJA, and temperature stress. In addition, the virus-induced NibenSFP1 silencing in tobacco and detected dramatically enhanced glucose content, indicating the NtSFP1 might regulate the glucose content and involved in MeJA signaling way to response the temperature stress. In general, our findings provide useful information on understanding the roles of STs in phytohormone signaling way and abiotic stresses in N. tabacum.
糖既是高等植物的营养物质，也是重要的信号分子。糖转运蛋白（STs）参与糖的加载和卸载，并促进跨膜的糖运输。烟草（Nicotiana tabacum）是一种模式植物，也是经济上最重要的植物之一。在我们的研究中，在烟草基因组中基于系统发育分析鉴定了 92 个烟草 ST（NtST）基因，并将其分为八个不同的亚家族。外显子-内含子分析表明，每个亚家族都表现出密切相关的基因结构特征，基于可比数量或长度的外显子。串联重复和纯化选择是 NtST 基因进化的主要因素。在 NtST 基因家族启动子序列中寻找顺式调控元件表明，它们可能受到光、植物激素和非生物胁迫因素的调控。我们在不同组织、各种非生物和植物激素胁迫下进行了全面的表达研究。结果揭示了 NtST 基因的不同表达模式和功能多样化。所得数据表明，NtSFP1 在所有测量的五种烟草组织中均高度表达，并且受 MeJA 和温度胁迫的调节。此外，病毒诱导的烟草中 NibenSFP1 的沉默导致葡萄糖含量显著增加，表明 NtSFP1 可能调节葡萄糖含量并参与 MeJA 信号途径以响应温度胁迫。总的来说，我们的研究结果提供了有关 ST 在植物激素信号途径和非生物胁迫中作用的有用信息。
