Department of Food Engineering, Hacettepe University, Beytepe, Turkey.
Department of Chemical Engineering, Hacettepe University, Beytepe, Turkey.
Prep Biochem Biotechnol. 2023;53(3):317-330. doi: 10.1080/10826068.2022.2084625. Epub 2022 Jun 17.
Hazelnut husk is one of the most abundant agricultural residue in Turkey. Valorization of this lignocellulosic biomass would provide a promoting alternative for economical production of pullulan. In this study, dried hazelnut husk hydrolysate was used directly as fermentation medium for pullulan production by a domestic strain of The aim of this work was the optimization of some fermentation medium parameters by central composite design using response surface methodology (RSM). The effects of (NH)SO concentration, the volume of concentrated HSO and the amount of ground hazelnut husk on pullulan production were optimized by RSM. The optimum levels of the fermentation parameters defined as 7.2 gL, 2.5 mL and 20 g, respectively. The maximum pullulan and exopolysaccharide concentrations were determined as 74.39 and 75.95 gL respectively in the optimum conditions. Specific growth rate of the strain was found as 0.097 h. FTIR spectral attributes confirmed the structure of pullulan. Thermal decomposition temperature of synthesized pullulan was found as 247.15 °C. This study showed that hazelnut husk was one of the novel substrate for production of the pullulan by AZ-6. No previous work was found to utilize dried hazelnut husk as fermentation medium for pullulan production by .
榛壳是土耳其最丰富的农业废弃物之一。这种木质纤维素生物质的增值利用将为经济生产普鲁兰提供一个有前途的替代方案。在这项研究中，直接使用干燥的榛壳水解物作为发酵培养基，由国内一株 生产普鲁兰。本工作的目的是通过响应面法（RSM）中的中心复合设计优化一些发酵培养基参数。通过 RSM 优化了 (NH)SO 浓度、浓 HSO 体积和榛壳粉用量对普鲁兰生产的影响。发酵参数的最佳水平分别定义为 7.2 gL、2.5 mL 和 20 g。在最佳条件下，普鲁兰和胞外多糖的最大浓度分别确定为 74.39 和 75.95 gL。菌株的比生长速率为 0.097 h。傅里叶变换红外光谱（FTIR）谱图特征证实了普鲁兰的结构。合成普鲁兰的热分解温度为 247.15°C。这项研究表明，榛壳是 通过 AZ-6 生产普鲁兰的新型底物之一。没有发现以前的工作利用干燥的榛壳作为发酵培养基生产普鲁兰。
