School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province 212013, China.
Pestic Biochem Physiol. 2022 Jun;184:105114. doi: 10.1016/j.pestbp.2022.105114. Epub 2022 May 6.
Previously, deltamethrin (a Type-II pyrethroid) has been reported to increase triglyceride (fat) accumulation in adipocytes, while its underlying molecular mechanism is not fully determined. The aim of this study was to further investigate the molecular mechanisms of deltamethrin induced fat accumulation in murine 3T3-L1 adipocytes. Consistent to previous reports, deltamethrin (10 μM) significantly promoted adipogenesis in 3T3-L1 adipocytes. RNA sequencing (RNA-seq) results showed that 721 differentially expressed genes (DEGs) were identified after deltamethrin treatment, involving in 58 Functional groups of Gene Ontology (GO) and 255 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Several key functional groups regulating adipogenesis, such as fat cell differentiation (Igf1, Snai2, Fgf10, and Enpp1) and cytosolic calcium ion concentration (Nos1, Cxcl1, and Ngf) were significantly enriched. Collectively, these results suggest that the promotion of adipogenesis by deltamethrin was attributed to an obesogenic global transcriptomic response, which provides further understanding of the underlying mechanisms of deltamethrin-induced fat accumulation.
先前有报道称，除虫菊酯（II 型拟除虫菊酯）可增加脂肪细胞中甘油三酯（脂肪）的积累，但其潜在的分子机制尚未完全确定。本研究旨在进一步探讨除虫菊酯诱导的小鼠 3T3-L1 脂肪细胞脂肪积累的分子机制。与先前的报道一致，除虫菊酯（10 μM）显著促进了 3T3-L1 脂肪细胞的脂肪生成。RNA 测序（RNA-seq）结果显示，除虫菊酯处理后鉴定出 721 个差异表达基因（DEGs），涉及 58 个基因本体论（GO）功能组和 255 个京都基因与基因组百科全书（KEGG）途径。几个关键的调节脂肪生成的功能组，如脂肪细胞分化（Igf1、Snai2、Fgf10 和 Enpp1）和细胞质钙离子浓度（Nos1、Cxcl1 和 Ngf）显著富集。综上所述，这些结果表明，除虫菊酯促进脂肪生成归因于肥胖相关的全局转录组反应，这为除虫菊酯诱导脂肪积累的潜在机制提供了进一步的理解。
