Karabekir Seda Cetinkaya, Sozen Mehmet Enes, Ayan Ilknur Cinar, Savas Hasan Basri, Cuce Gokhan, Kalkan Serpil
Izmir Bakırcay University, Faculty of Medicine, Department of Histology and Embryology, Izmir, Turkey.
Alanya Alaaddin Keykubat University, Faculty of Medicine, Department of Histology and Embryology, Alanya, Turkey.
Iran J Basic Med Sci. 2025;28(7):929-936. doi: 10.22038/ijbms.2025.82598.17854.
Deltamethrin (DLM) is a widely used insecticide in agriculture; however, exposure to it can lead to serious health problems. This study aimed to evaluate the protective effects of hesperidin (HSP), a natural antioxidant, against DLM-induced liver toxicity.
Thirty-two male Wistar Albino rats (250-300 g, 4 months old) were divided into four groups. The control group received 1 ml of corn oil via oral gavage for 30 days. The DLM group received 1.28 mg/kg DLM in corn oil for 30 days. The DLM+HSP 100 mg/kg and DLM+HSP 300 mg/kg groups received 1.28 mg/kg DLM followed by 100 mg/kg or 300 mg/kg HSP in distilled water, respectively, 30 min after DLM administration for 30 days. Liver tissues were examined histopathologically. Masson's trichrome staining and PCR assessed fibrosis. Caspase 3 and 9 expressions in liver tissues were determined by immunohistochemistry and PCR. Biochemical analyses were conducted on serum samples.
HSP supplementation led to a dose-dependent decrease in aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. DLM exposure decreased antioxidant capacity, while HSP supplementation increased it dose-dependently. Histopathological evaluations showed increased liver damage in the DLM group, while HSP administration reduced liver toxicity. Masson's trichrome staining and analysis of collagen I (COL1A1) and collagen III (COL3A1) gene expression revealed increased fibrosis in the DLM group, which was attenuated with HSP treatment.
The potential prevention of DLM-induced liver toxicity and apoptosis by HSP may be an alternative protective strategy.
溴氰菊酯（DLM）是一种在农业中广泛使用的杀虫剂；然而，接触它会导致严重的健康问题。本研究旨在评估天然抗氧化剂橙皮苷（HSP）对DLM诱导的肝毒性的保护作用。
将32只雄性Wistar白化大鼠（250 - 300克，4个月大）分为四组。对照组经口灌胃1毫升玉米油，持续30天。DLM组经口灌胃1.28毫克/千克DLM溶于玉米油中，持续30天。DLM + HSP 100毫克/千克组和DLM + HSP 300毫克/千克组在给予DLM 30分钟后，分别经口给予1.28毫克/千克DLM，随后给予100毫克/千克或300毫克/千克HSP溶于蒸馏水中，持续治疗30天。对肝组织进行组织病理学检查。采用Masson三色染色法和PCR评估纤维化程度。通过免疫组织化学和PCR测定肝组织中半胱天冬酶3和9的表达。对血清样本进行生化分析。
补充HSP导致天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平呈剂量依赖性降低。接触DLM会降低抗氧化能力，而补充HSP会使其剂量依赖性增加。组织病理学评估显示，DLM组肝损伤增加，而给予HSP可降低肝毒性。Masson三色染色以及对I型胶原蛋白（COL1A1）和III型胶原蛋白（COL3A1）基因表达的分析显示，DLM组纤维化增加，而HSP治疗可减轻纤维化。
HSP对DLM诱导的肝毒性和细胞凋亡的潜在预防作用可能是一种替代性保护策略。
