Medicinal Chemistry Theme, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria 3052, Australia.
School of Natural & Environmental Sciences, Newcastle University, Newcastle Upon Tyne NE1 7RU, U.K.
Bioconjug Chem. 2024 Jun 19;35(6):715-731. doi: 10.1021/acs.bioconjchem.4c00084. Epub 2024 May 22.
Click chemistry has become a commonly used synthetic method due to the simplicity, efficiency, and high selectivity of this class of chemical reactions. Since their initial discovery, further click chemistry methods have been identified and added to the toolbox of click chemistry reactions for biomedical applications. However, selecting the most suitable reaction for a specific application is often challenging, as multiple factors must be considered, including selectivity, reactivity, biocompatibility, and stability. Thus, this review provides an overview of the benefits and limitations of well-established click chemistry reactions with a particular focus on the importance of considering reaction rates, an often overlooked criterion with little available guidance. The importance of understanding each click chemistry reaction beyond simply the reaction speed is discussed comprehensively with reference to recent biomedical research which utilized click chemistry. This review aims to provide a practical resource for researchers to guide the selection of click chemistry classes for different biomedical applications.
点击化学因其反应的简单性、高效性和高选择性，已成为一种常用的合成方法。自最初发现以来，已经确定了更多的点击化学方法，并将其添加到用于生物医学应用的点击化学反应工具箱中。然而，选择最适合特定应用的反应通常具有挑战性，因为必须考虑多个因素，包括选择性、反应性、生物相容性和稳定性。因此，本综述概述了经过充分验证的点击化学反应的优点和局限性，特别强调了考虑反应速率的重要性，这是一个经常被忽视的标准，几乎没有可用的指导。本综述通过参考最近利用点击化学的生物医学研究，全面讨论了超越简单反应速度来理解每个点击化学反应的重要性。本综述旨在为研究人员提供实用的资源，指导他们选择不同的点击化学类用于不同的生物医学应用。
