Tu Li-Chan, Cai Xin-Bo, Gao Jie, Tu Yu-He, Liu San-Bo, Gao Wei
Beijing Shijitan Hospital, Capital Medical University Beijing 100038, China School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China School of Medicine, Zhejiang University City College Hangzhou 310015, China.
School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
Zhongguo Zhong Yao Za Zhi. 2022 Jun;47(11):2909-2914. doi: 10.19540/j.cnki.cjcmm.20220215.101.
The seeds of Tripterygium wilfordii are characterized by dormancy and a long germination cycle under natural sowing conditions. In this study, we developed a method for rapid germination of T. wilfordii seeds by analyzing the size, morphology, thousand-grain weight, viability, moisture content, physicochemical properties, and seed germination rates under different germination conditions. The seeds of T. wilfordii were fine columnar with a thick and hard outer seed coat. They had the length of 6.69 mm, the width of 2.14 mm, the thickness of 1.68 mm, the thousand-grain weight of 8.99 g, the moisture content of 8.86%, the soluble sugar content of 21.3 mg·g(-1), the starch content of 28.9 mg·g(-1), the soluble protein content of 44.2 mg·g(-1), and the seed viability of only 54.0%. The seeds were respectively treated with distilled water, ultrasonication, low-temperature storage, 50 ℃ water, 100 mg·L(-1) 6-BA, 0.6% KMnO_4, 1% KNO_3, 50 mg·L(-1) NAA, and 100 mg·L(-1) GA_3 solution. The results showed that soaking the seeds in 100 mg·L(-1) GA_3 solution significantly promoted the germination. Further, the seeds were soaked in 50, 100, 250, 500, and 1 000 mg·L(-1) GA_3 solutions, which demonstrated that high concentration(500 mg·L(-1), 1 000 mg·L(-1)) of GA_3 solutions increased the germination rate and speed and shortened the germination cycle from more than 3 months to less than 15 days. The findings of this study are of great significance to the breeding of T. wilfordii and lay a foundation for the large-scale propagation of T. wilfordii seeds and the excavation of T. wilfordii germplasm resources.
雷公藤种子具有休眠特性，在自然播种条件下萌发周期较长。在本研究中，我们通过分析雷公藤种子的大小、形态、千粒重、活力、含水量、理化性质以及不同萌发条件下的种子萌发率，开发了一种雷公藤种子快速萌发的方法。雷公藤种子呈细柱状，外种皮厚且硬。其长度为6.69毫米，宽度为2.14毫米，厚度为1.68毫米，千粒重为8.99克，含水量为8.86%，可溶性糖含量为21.3毫克·克⁻¹，淀粉含量为28.9毫克·克⁻¹，可溶性蛋白含量为44.2毫克·克⁻¹，种子活力仅为54.0%。种子分别用蒸馏水、超声处理、低温贮藏、50℃水、100毫克·升⁻¹ 6 - BA、0.6% KMnO₄、1% KNO₃、50毫克·升⁻¹ NAA和100毫克·升⁻¹ GA₃溶液处理。结果表明，将种子浸泡在100毫克·升⁻¹ GA₃溶液中显著促进了萌发。此外，将种子浸泡在50、100、250、500和1000毫克·升⁻¹ GA₃溶液中，结果表明高浓度（500毫克·升⁻¹、1000毫克·升⁻¹）的GA₃溶液提高了萌发率和速度，将萌发周期从3个多月缩短至不到15天。本研究结果对雷公藤育种具有重要意义，为雷公藤种子的大规模繁殖和雷公藤种质资源的挖掘奠定了基础。
