Sun Haiyan, Li Yan, Si Wengang, Hua Tian, Chen Juan, Kang Shan
Department of Obstetrics and Gynecology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Department of Molecular Biology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Front Oncol. 2022 May 26;12:763134. doi: 10.3389/fonc.2022.763134. eCollection 2022.
This study aims to investigate the effect of polymorphisms of programmed cell death-ligand 1 (PD-L1) on the risk and patient's outcomes of epithelial ovarian cancer (EOC).
Totally, 568 patients and 532 healthy women were included. Three polymorphisms in the PD-L1 gene, rs2297136, rs4143815 and rs4742098, were genotyped by the polymerase chain reaction/ligase detection reaction (PCR-LDR). Survival analysis was performed in 234 patients (received primary debulking surgery followed by platinum-based chemotherapy).
Patients with the rs2297136 AG + GG genotypes had shorter progression-free survival (PFS) (hazard ratio (HR)=1.44, 95% CI=1.03-2.01) and overall survival (OS) (HR=1.55, 95% CI=1.06-2.27) than those with the AA genotype. Moreover, the mRNA and protein expression levels of PD-L1 in EOC tissues with the rs2297136 AG + GG genotypes were remarkably higher than those with the AA genotype (P=0.032 and P=0.047, respectively). Survival analysis showed that high expression of PD-L1 mRNA was remarkably associated with worse 10-year PFS (HR=1.55, 95% CI=1.28-1.88) and OS (HR=1.51, 95% CI=1.00-2.28) in EOC patients.
The rs2297136 may not only effectively influence the expression of PD-L1, but also is significantly associated with EOC patients' outcomes.
本研究旨在探讨程序性细胞死亡配体1（PD-L1）基因多态性对上皮性卵巢癌（EOC）发病风险及患者预后的影响。
共纳入568例患者和532名健康女性。采用聚合酶链反应/连接酶检测反应（PCR-LDR）对PD-L1基因的3个多态性位点rs2297136、rs4143815和rs4742098进行基因分型。对234例接受了初次肿瘤细胞减灭术并随后接受铂类化疗的患者进行生存分析。
rs2297136位点AG + GG基因型患者的无进展生存期（PFS）（风险比（HR）=1.44，95%置信区间（CI）=1.03 - 2.01）和总生存期（OS）（HR=1.55，95% CI=1.06 - 2.27）均短于AA基因型患者。此外，rs2297136位点AG + GG基因型的EOC组织中PD-L1的mRNA和蛋白表达水平显著高于AA基因型（分别为P = 0.032和P = 0.047）。生存分析显示，EOC患者中PD-L1 mRNA高表达与10年PFS（HR=1.55，95% CI=1.28 - 1.88）和OS（HR=1.51，95% CI=1.00 - 2.28）显著相关。
rs2297136不仅可能有效影响PD-L1的表达，还与EOC患者的预后显著相关。
