Bae Hyun-Gyeong, Kim Mi-Ja
Department of Food and Nutrition, Kangwon National University, Samcheok, Gangwon 25949 Republic of Korea.
Food Sci Biotechnol. 2022 Apr 27;31(7):879-892. doi: 10.1007/s10068-022-01086-z. eCollection 2022 Jul.
Buckwheat germinated on days of 3, 5, and 7 was digested in vitro, and the antioxidant and anti-obesity effects of the digesta were evaluated. In vitro digesta of 5 days germinated buckwheat (GBD5) showed significantly higher antioxidant activity in DPPH, ABTS, total phenolic content, total flavonoid content, and ferric reducing antioxidant power by 5.3, 1.3, 2.0, 3.2, and 2.8-fold, respectively than in vitro digesta of non-germinated buckwheat. GBD5 exerted inhibitory effect on total lipid accumulation in 3T3-L1 adipocyte in a dose-dependent manner, with over 25% reduction at 400 µg/mL. Additionally, GBD5 significantly downregulated genes related to adipocyte differentiation and fat accumulation. GBD5 possessed different metabolite profiles compared to others such as higher content of γ-aminobutyric acid and succinic acid. Therefore, GBD5 has potent antioxidant effects and suppresses fat accumulation-related genes and proteins expression, which could act as a new functional substance.
对在第3、5和7天发芽的荞麦进行体外消化，并评估消化产物的抗氧化和抗肥胖作用。5天发芽荞麦的体外消化产物（GBD5）在DPPH、ABTS、总酚含量、总黄酮含量和铁还原抗氧化能力方面的抗氧化活性分别比未发芽荞麦的体外消化产物高5.3倍、1.3倍、2.0倍、3.2倍和2.8倍。GBD5对3T3-L1脂肪细胞中的总脂质积累具有剂量依赖性抑制作用，在400μg/mL时减少超过25%。此外，GBD5显著下调了与脂肪细胞分化和脂肪积累相关的基因。与其他物质相比，GBD5具有不同的代谢物谱，如γ-氨基丁酸和琥珀酸含量较高。因此，GBD5具有强大的抗氧化作用，可抑制脂肪积累相关基因和蛋白质的表达，有望成为一种新型功能物质。
