Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China.
Laboratory of Fruit Quality Biology, Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou 310058, China.
Food Chem. 2018 Aug 30;258:43-50. doi: 10.1016/j.foodchem.2018.03.049. Epub 2018 Mar 13.
This research aimed to investigate the protective effects of a new mulberry cultivar J33 with simulated gastrointestinal digestion against palmitic acid (PA)-induced lipotoxicity. LC-MS analysis revealed that the contents of four flavonoid glycosides (quercetin rhamnosylhexoside hexoside, quercetin rhamnosylhexoside, quercetin hexoside, kaempferol rhamnosylhexoside) increased after digestion. Besides, mulberry digest (MBD) at 0.5-2 mg/mL significantly reduced PA-induced lipotoxicity in human hepatocytes, while mulberry extract without digestion (MBE) showed no protection. Further investigations demonstrated that the protection of MBD was attributed to two aspects. On the one hand, MBD could attenuate PA-induced oxidative stress by suppressing ROS accumulation, regulating intracellular glutathione and ameliorating mitochondrial dysfunction. On the other hand, MBD could promote PA incorporation into inert triglycerides (TG) to deal with the acute lipid overload, reducing the lipotoxicity caused by PA. Overall, our research might provide a new perspective of mulberry cultivar J33 in ameliorating non-alcoholic fatty liver disease (NAFLD).
本研究旨在探究具有模拟胃肠道消化特性的新型桑品种 J33 对棕榈酸（PA）诱导的脂毒性的保护作用。LC-MS 分析显示，四种类黄酮糖苷（芦丁鼠李糖苷半乳糖苷、芦丁鼠李糖苷、芦丁葡萄糖苷、山柰酚鼠李糖苷）的含量在消化后增加。此外，0.5-2mg/mL 的桑椹消化物（MBD）可显著减轻 PA 诱导的人肝细胞脂毒性，而未经消化的桑椹提取物（MBE）则没有保护作用。进一步的研究表明，MBD 的保护作用归因于两个方面。一方面，MBD 可以通过抑制 ROS 积累、调节细胞内谷胱甘肽和改善线粒体功能来减轻 PA 诱导的氧化应激。另一方面，MBD 可以促进 PA 掺入惰性三酰基甘油（TG）中以应对急性脂质过载，从而减少 PA 引起的脂毒性。总体而言，本研究可能为改善非酒精性脂肪性肝病（NAFLD）提供了新型桑品种 J33 的新视角。
