Yin Chengzeng, Okugawa Yoshinaga, Yamamoto Akira, Kitajima Takahito, Shimura Tadanobu, Kawamura Mikio, Tsujiura Masahiro, Okita Yosiki, Ohi Masaki, Toiyama Yuji
Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan.
Department of Genomic Medicine, Mie University Hospital, Tsu, Mie 514-8507, Japan.
Oncol Lett. 2022 May 17;24(1):212. doi: 10.3892/ol.2022.13333. eCollection 2022 Jul.
Tumor-infiltrating immune cells play an essential role in cancer progression and may help supplement the Tumor, Node, Metastasis (TNM) classification for cancer prognosis. Currently, there are numerous conflicting reports discussing the significance of tumor-associated neutrophils (TANs) in colorectal cancer (CRC). In particular, the role of TANs in the invasive margin is unclear. The present study investigated the prognostic significance of CD66 TANs and CD8 tumor-infiltrating lymphocytes (TILs) in the invasive margin of 103 patients with CRC. By using immunohistochemistry, survival analysis was performed on CD8 TILs and CD66 TANs individually, as well as models including TILs and TANs simultaneously. The findings indicated that the densities of CD8 TILs and CD66b TANs in the invasive margin may provide significant prognostic value for predicting survival. Moreover, the combined evaluation of CD8 TILs and CD66b TANs in the invasive margin could further improve the validity for the prediction of oncological outcomes. In addition, multivariate analysis revealed that simultaneous low tumor infiltration by CD8 TILs and CD66b was an independent predictive factor for overall survival (HR=4.17, 95% CI, 1.55-12.5; P=0.004) and disease-free survival (HR=2.75, 95% CI, 1.27-6.12; P=0.01). Given the importance of CD8 TILs and CD66b TANs in the tumor microenvironment, the assessment of their densities in the invasive margin may serve as a valuable prognostic marker for CRC.
肿瘤浸润免疫细胞在癌症进展中起着至关重要的作用，可能有助于补充肿瘤、淋巴结、转移（TNM）分类以用于癌症预后评估。目前，有许多相互矛盾的报道讨论了肿瘤相关中性粒细胞（TANs）在结直肠癌（CRC）中的意义。特别是，TANs在浸润边缘的作用尚不清楚。本研究调查了103例CRC患者浸润边缘中CD66 TANs和CD8肿瘤浸润淋巴细胞（TILs）的预后意义。通过免疫组织化学方法，分别对CD8 TILs和CD66 TANs进行生存分析，同时也对包括TILs和TANs的模型进行了分析。研究结果表明，浸润边缘中CD8 TILs和CD66b TANs的密度可能为预测生存提供显著的预后价值。此外，对浸润边缘的CD8 TILs和CD66b TANs进行联合评估可进一步提高肿瘤学结局预测的有效性。此外，多因素分析显示，CD8 TILs和CD66b同时低肿瘤浸润是总生存（HR = 4.17，95%CI，1.55 - 12.5；P = 0.004）和无病生存（HR = 2.75，95%CI，1.27 - 6.12；P = 0.01）的独立预测因素。鉴于CD8 TILs和CD66b TANs在肿瘤微环境中的重要性，评估它们在浸润边缘的密度可能作为CRC的一个有价值的预后标志物。
