基因提高[具体对象]的耐旱性。 （注：原文中“in.”后面内容缺失）
Genes Improve Drought Tolerance in .
作者信息
Zhang Yongli, Du Pei, Xiong Faqian, Zhang Xiaojun, Song Hui
机构信息
Grassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao, China.
Industrial Crops Research Institute, Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement, Zhengzhou, China.
出版信息
Front Plant Sci. 2022 May 26;13:910408. doi: 10.3389/fpls.2022.910408. eCollection 2022.
WRKY transcription factor participates in plant growth and development and response to biotic and abiotic stresses. , a turfgrass, has high drought tolerance, yet little is known about response to drought stress in . In this study, RNA-seq identified five , including , , , , and , which were upregulated under drought stress. Orthologous relationships between and were determined to predict the regulatory networks of the five AdWRKYs based on AtWRKYs. Additionally, protein-protein interactions were predicted using differentially expressed proteins from RNA-seq. The quantitative real-time PCR (qRT-PCR) results showed that was upregulated, while , , and were downregulated at different time-points under drought stress. The predicted regulatory networks showed that AdWRKY40 activates , , and expression under drought stress. Besides, AdWRKY56 regulated under drought stress. Aradu.YIQ80 (NAC019) interacted with AdWRKY40, AdWRKY42, AdWRKY56, and AdWRKY64, while Aradu.Z5H58 (NAC055) interacted with AdWRKY42 and AdWRKY64 under drought stress. This study used to assess function and regulatory networks, providing a basis for understanding drought tolerance in .
WRKY转录因子参与植物的生长发育以及对生物和非生物胁迫的响应。狗牙根是一种草坪草，具有较高的耐旱性，但关于狗牙根对干旱胁迫的响应了解甚少。在本研究中，RNA测序鉴定出五个AdWRKY基因，包括AdWRKY40、AdWRKY42、AdWRKY56、AdWRKY64和AdWRKY71，它们在干旱胁迫下上调。基于拟南芥WRKY基因确定了狗牙根与拟南芥之间的直系同源关系，以预测这五个AdWRKY基因的调控网络。此外，利用RNA测序中差异表达的蛋白质预测了蛋白质-蛋白质相互作用。定量实时PCR（qRT-PCR）结果表明，在干旱胁迫下不同时间点，AdWRKY40上调，而AdWRKY42、AdWRKY56和AdWRKY64下调。预测的调控网络显示，干旱胁迫下AdWRKY40激活AdWRKY42、AdWRKY56和AdWRKY64的表达。此外，干旱胁迫下AdWRKY56调控AdWRKY64。在干旱胁迫下，Aradu.YIQ80（NAC019）与AdWRKY40、AdWRKY42、AdWRKY56和AdWRKY64相互作用，而Aradu.Z5H58（NAC055）与AdWRKY42和AdWRKY64相互作用。本研究利用狗牙根评估AdWRKY基因的功能和调控网络，为了解狗牙根的耐旱性提供了依据。
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