Lee Hyun Ji, Kwack Mi Hee, Lee Weon Ju
Department of Dermatology, School of Medicine, Kyungpook National University, Daegu, Korea.
Department of Immunology, School of Medicine, Kyungpook National University, Daegu, Korea.
Ann Dermatol. 2021 Oct;33(5):409-418. doi: 10.5021/ad.2021.33.5.409. Epub 2021 Sep 8.
Although ginseng has beneficial effects largely related to their constituent ginsenosides, pharmacological effects of non-ginsenosides have been reported. Acidic polysaccharides of red ginseng (RGAP) are among the non-ginsenoside constituents that have characterized antioxidant properties.
We investigated the impact of RGAP on sebocytes and outer root sheath (ORS) cells treated with lipopolysaccharide (LPS) and in mice with ()-induced inflammatory nodules.
Sebocytes and ORS cells were cultured and treated with either 0.1% dimethyl sulfoxide, 5 g/ml LPS, 50 g/ml RGAP or 5 g/ml LPS+50 g/ml RGAP for 6 and 24 hours. Real-time polymerase chain reaction, ELISA, Western blot analysis, and immunofluorescence staining were among the methods used to detect and quantify inflammatory cytokine production. Mice infected with were treated with 2 weeks of RGAP provided in drinking water followed by immunohistochemical evaluation of inflammatory nodules.
Administration of RGAP to LPS-treated sebocytes and ORS cell cultures resulted in increased expression of inflammatory cytokines like interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α, toll-like receptor 2, p-c-jun, p-JNK and p-iKB (<0.05). Administration of RGAP also resulted in increased expression of LL37 in LPS-treated sebocytes and ORS cells, and increased production of sebum in LPS-treated sebocytes (<0.05). RGAP also promoted increased expression of inflammatory biomarkers in -associated inflammatory nodules in mice (<0.05).
RGAP may exacerbate inflammatory pathology associated with acne vulgaris. Ginseng supplements may be contraindicated in patients diagnosed with inflammatory acne.
尽管人参的有益作用很大程度上与其成分人参皂苷有关，但非人参皂苷的药理作用也有报道。红参酸性多糖（RGAP）是具有抗氧化特性的非人参皂苷成分之一。
我们研究了RGAP对用脂多糖（LPS）处理的皮脂腺细胞和外根鞘（ORS）细胞以及对（）诱导的炎性结节小鼠的影响。
培养皮脂腺细胞和ORS细胞，并用0.1%二甲基亚砜、5μg/ml LPS、50μg/ml RGAP或5μg/ml LPS + 50μg/ml RGAP处理6小时和24小时。实时聚合酶链反应、酶联免疫吸附测定、蛋白质印迹分析和免疫荧光染色是用于检测和定量炎性细胞因子产生的方法之一。用饮用水中提供的RGAP处理感染的小鼠2周，然后对炎性结节进行免疫组织化学评估。
将RGAP给予经LPS处理的皮脂腺细胞和ORS细胞培养物导致炎性细胞因子如白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子-α、Toll样受体2、p-c-jun、p-JNK和p-IκB的表达增加（<0.05）。给予RGAP还导致经LPS处理的皮脂腺细胞和ORS细胞中LL37的表达增加，以及经LPS处理的皮脂腺细胞中皮脂分泌增加（<0.05）。RGAP还促进小鼠中与相关炎性结节中炎性生物标志物的表达增加（<0.05）。
RGAP可能会加重寻常痤疮相关的炎性病理。在被诊断为炎性痤疮的患者中，人参补充剂可能是禁忌的。
