Lee Mon-Chien, Hsu Yi-Ju, Wu Fang-Yu, Huang Chi-Chang, Li Hsueh-Yu, Chen Wen-Chyuan
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan, Taiwan.
Department of Otorhinolaryngology-Head and Neck Surgery, Sleep Center, Linkou-Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Front Physiol. 2022 May 26;13:893352. doi: 10.3389/fphys.2022.893352. eCollection 2022.
In recent years, the aging population has gradually increased, and the aging process is accompanied by health-associated problems, such as loss of muscle mass and weakness. Therefore, it is important to explore alternative strategies for improving the health status and physical fitness of the aged population. In this study, we investigated the effect of soy protein supplementation combined with resistance training on changes in the muscle mass, muscle strength, and functional activity performance of aging mice. Female Institute of Cancer Research (ICR) mice were divided into four groups ( = 8 per group): sedentary control (SC), isolated soy protein (ISP) supplementation, resistance training (RT), and a combination of ISP and RT (ISP + RT). The mice in designated groups received oral ISP supplementation (0.123 g/kg/day), RT (5 days/week for a period of 4 weeks), or a combination of both ISP plus RT for 4 weeks. Afterward, we assessed muscle strength, endurance, and anaerobic endurance performance and analyzed blood biochemical and pathological tissue sections to investigate whether there were adverse effects or not in mice. ISP supplementation effectively improved the muscle mass, muscle endurance, and endurance performance of aging female mice. The RT group not only showed similar results with ISP but also increased muscle strength and glycogen content. Nevertheless, the combination of ISP supplementation and RT had greater beneficial effects on muscle strength, physical performance, and glycogen levels ( < 0.05). In addition, the combination of ISP supplementation and RT had significantly increased type II muscle percentage and cross-sectional area ( < 0.05). Although ISP or RT alone improved muscle mass and performance, the combination of ISP with RT showed greater beneficial effects in aging mice. Our findings suggest that regular exercise along with protein supplementation could be an effective strategy to improve overall health and physical fitness among the elderly.
近年来，老年人口逐渐增加，衰老过程伴随着与健康相关的问题，如肌肉量减少和虚弱。因此，探索改善老年人群健康状况和身体素质的替代策略很重要。在本研究中，我们调查了补充大豆蛋白结合抗阻训练对衰老小鼠肌肉量、肌肉力量和功能活动表现变化的影响。将雌性癌症研究所（ICR）小鼠分为四组（每组 = 8只）：久坐对照组（SC）、补充分离大豆蛋白（ISP）组、抗阻训练（RT）组以及ISP与RT联合组（ISP + RT）。指定组的小鼠接受口服ISP补充（0.123 g/kg/天）、RT（每周5天，持续4周）或ISP加RT联合干预4周。之后，我们评估了肌肉力量、耐力和无氧耐力表现，并分析了血液生化指标和病理组织切片，以研究小鼠是否存在不良反应。补充ISP有效改善了衰老雌性小鼠的肌肉量、肌肉耐力和耐力表现。RT组不仅显示出与ISP组相似的结果，还增加了肌肉力量和糖原含量。然而，补充ISP与RT联合组对肌肉力量、身体表现和糖原水平有更大的有益影响（P < 0.05）。此外，补充ISP与RT联合组的II型肌百分比和横截面积显著增加（P < 0.05）。虽然单独的ISP或RT改善了肌肉量和表现，但ISP与RT联合组对衰老小鼠显示出更大的有益影响。我们的研究结果表明，定期运动结合蛋白质补充可能是改善老年人整体健康和身体素质的有效策略。
