Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan.
Department of Orthopedic Surgery, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan.
Nutrients. 2018 Nov 17;10(11):1792. doi: 10.3390/nu10111792.
Isolated soy protein (ISP) is a well-known supplement and has been reported to improve health, exercise performance, body composition, and energy utilization. ISP exhibits multifunctional bioactivities and also contains branched-chain amino acids (BCAAs), which have been confirmed to positively affect body weight (BW) regulation and muscle protein synthesis. The combined effects of BCAA supplements and exercise in older postmenopausal women with osteoporosis, sarcopenia, and obesity have been inadequately investigated. Therefore, in this study, we evaluated the potential beneficial effects of soy protein supplementation and exercise training on postmenopausal mice. Forty mice (14 weeks old) with ovariectomy-induced osteosarcopenic obesity were divided into five groups ( = 8), namely sham ovariectomy (OVX, control), OVX, OVX with ISP supplementation (OVX+ISP), OVX with exercise training (ET, OVX+ET), and OVX with ISP and ET (OVX+ISP+ET). The mice received a vehicle or soy protein (3.8 g/kg BW) by oral gavage for four weeks, and the exercise performance (forelimb grip strength and exhaustive swimming time) was evaluated. In the biochemical profiles, we evaluated the serum glucose level and tissue damage markers, such as lactate, ammonia, glucose, blood urine nitrogen (BUN), and creatinine phosphate kinase (CPK). The body composition was determined by evaluating bone stiffness and muscle mass. All data were analyzed using one-way repeated measures analysis of variance. The physical performance of the OVX+ISP+ET group did not differ from that of the other groups. The OVX+ISP+ET group exhibited lower levels of serum lactate, ammonia, CPK, and BUN as well as economized glucose metabolism after an acute exercise challenge. The OVX+ISP+ET group also exhibited higher muscle mass and bone strength than the OVX group. Our study demonstrated that a combination of ISP supplementation and exercise reduced fatigue and improved bone function in OVX mice.
大豆分离蛋白（ISP）是一种众所周知的补充剂，已被报道可改善健康、运动表现、身体成分和能量利用。ISP 具有多种功能活性，还含有支链氨基酸（BCAAs），已被证实对体重（BW）调节和肌肉蛋白质合成有积极影响。BCAA 补充剂和运动对患有骨质疏松症、肌肉减少症和肥胖症的绝经后老年女性的联合影响尚未得到充分研究。因此，在这项研究中，我们评估了大豆蛋白补充剂和运动训练对绝经后小鼠的潜在有益作用。四十只（14 周龄）去卵巢诱导的骨质疏松性肌肉减少症肥胖小鼠被分为五组（每组 8 只），即假手术去卵巢（OVX，对照组）、OVX、OVX 加 ISP 补充剂（OVX+ISP）、OVX 加运动训练（ET，OVX+ET）和 OVX 加 ISP 和 ET（OVX+ISP+ET）。小鼠通过口服灌胃接受载体或大豆蛋白（3.8 g/kg BW），持续四周，并评估运动表现（前肢握力和力竭游泳时间）。在生化谱中，我们评估了血清葡萄糖水平和组织损伤标志物，如乳酸、氨、葡萄糖、血尿素氮（BUN）和肌酸磷酸激酶（CPK）。通过评估骨硬度和肌肉质量来确定身体成分。所有数据均使用单向重复测量方差分析进行分析。OVX+ISP+ET 组的身体表现与其他组没有差异。OVX+ISP+ET 组在急性运动挑战后表现出较低的血清乳酸、氨、CPK 和 BUN 水平，以及葡萄糖代谢的经济性。与 OVX 组相比，OVX+ISP+ET 组还表现出更高的肌肉质量和骨强度。我们的研究表明，ISP 补充剂和运动的结合减少了 OVX 小鼠的疲劳并改善了骨功能。
