Gharehaghaji Nahideh, Divband Baharak
Department of Radiology, Faculty of Allied Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Evid Based Complement Alternat Med. 2022 May 27;2022:1337588. doi: 10.1155/2022/1337588. eCollection 2022.
The design of new magnetic resonance imaging (MRI) contrast media with chemotherapy drug-carrying capacity has an important role in diagnostic and therapeutic purposes. This study aimed to synthesize a polyethylene glycol (PEG)-coated magnetite/hydroxyapatite nanocomposite as an MRI contrast agent investigate its curcumin loading/release properties and consider the cytotoxicity effect of the curcumin-loaded nanocomposite on different cell lines.
PEGylated magnetite/hydroxyapatite (PMHA) nanocomposite was synthesized and characterized using -ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, vibrating sample magnetometry, and energy dispersive -ray analysis. MTT assay was performed to consider the A549, MCF-7, and MRC-5 cells toxicity of the PMHA and the curcumin-loaded nanocomposite. The r2 relaxivity of the nanocomposite was determined by an MRI device. The curcumin loading and its release from the nanocomposite at pH of 7.4 and 5.5 were investigated.
The spherical nanocomposite showed an average size of 20 nm and a superparamagnetic property. PMHA nanocomposite was highly cytocompatible, while the curcumin-loaded nanocomposite showed significant cytotoxicity for A549 and a much higher toxic effect on MCF-7 cancer cells. The r2 relaxivity was measured as 120 mMS. The curcumin loading capacity of PMHA was 1.9 mg/g, and the curcumin release profile showed a pH-dependent sustained release of the anti-cancer drug that was higher for pH of 5.5.
The high r2 relaxivity of PMHA nanocomposite and sustained release of curcumin from the loaded one at the pH of tumor environment suggest that the nanocomposite is a potential candidate for T2-weighted MRI and cancer treatment.
设计具有化疗药物携带能力的新型磁共振成像（MRI）造影剂在诊断和治疗方面具有重要作用。本研究旨在合成一种聚乙二醇（PEG）包覆的磁铁矿/羟基磷灰石纳米复合材料作为MRI造影剂，研究其姜黄素负载/释放特性，并考察负载姜黄素的纳米复合材料对不同细胞系的细胞毒性作用。
合成了聚乙二醇化磁铁矿/羟基磷灰石（PMHA）纳米复合材料，并通过X射线衍射、傅里叶变换红外光谱、透射电子显微镜、振动样品磁强计和能量色散X射线分析对其进行表征。采用MTT法考察PMHA和负载姜黄素的纳米复合材料对A549、MCF-7和MRC-5细胞的毒性。通过MRI设备测定纳米复合材料的r2弛豫率。研究了纳米复合材料在pH值为7.4和5.5时姜黄素的负载及其释放情况。
球形纳米复合材料的平均尺寸为20nm，具有超顺磁性。PMHA纳米复合材料具有高度的细胞相容性，而负载姜黄素的纳米复合材料对A549显示出显著的细胞毒性，对MCF-7癌细胞的毒性作用更高。r2弛豫率测定为120mMS。PMHA的姜黄素负载量为1.9mg/g，姜黄素释放曲线显示抗癌药物在pH值为5.5时具有更高的pH依赖性缓释。
PMHA纳米复合材料的高r2弛豫率以及负载姜黄素的纳米复合材料在肿瘤环境pH值下姜黄素的缓释表明，该纳米复合材料是T2加权MRI和癌症治疗的潜在候选材料。
