Divband Baharak, Haleem Al-Qaim Zahraa, Hussein Falah H, Khezerloo Davood, Gharehaghaji Nahideh
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Inorganic Chemistry Department, Chemistry Faculty, University of Tabriz, Tabriz, Iran.
J Biomed Phys Eng. 2024 Dec 1;14(6):533-546. doi: 10.31661/jbpe.v0i0.2403-1736. eCollection 2024 Dec.
Application of the nanomaterials to preparing X-ray shields and successfully treating multiresistant microorganisms has attracted great attention in modern life.
This study aimed to prepare flexible silicone-based matrices containing BiO, PbO, or BiO/PbO nanoparticles and select a cost-effective, cytocompatible, and antibacterial/antifungal X-ray shield in clinical radiography.
In this experimental study, we prepared the nanoparticles by the modified biosynthesis method and fabricated the X-ray shields containing 20 wt% of the nanoparticles. The X-ray attenuation percentage and Half Value Layer (HVL) of the shields were investigated for the photon energies in the range of 40-100 kVp in clinical radiography. The antibacterial/antifungal activities of the shields were evaluated using a colony count method for the gram-negative (), and gram-positive () bacteria, and Candida albicans fungus. The shield toxicity was investigated on A549 cells.
The highest X-ray attenuation percentage and the lowest HVL were obtained using the shield containing BiO nanoparticles. Although all shields displayed antimicrobial activity, the shield containing BiO/PbO nanoparticles showed the most effective reduction in the colony counts. Both X-ray shields containing nano BiO and BiO/PbO demonstrated high cytocompatibility on A549 cells at a concentration as high as 500 µg/ml. The shield with PbO nanoparticles was also cytocompatible at a concentration of 50 µg/ml.
The best X-ray attenuation performance is attributed to the silicone-based matrix with nano BiO; however, the flexible shield with BiO/PbO nanoparticles can be cost-effective and cytocompatible with the best antibacterial/antifungal properties.
纳米材料在制备X射线防护材料以及成功治疗多重耐药微生物方面的应用在现代生活中引起了极大关注。
本研究旨在制备含有BiO、PbO或BiO/PbO纳米粒子的柔性有机硅基基质，并在临床放射摄影中选择一种经济高效、细胞相容性好且具有抗菌/抗真菌性能的X射线防护材料。
在本实验研究中，我们通过改良的生物合成方法制备了纳米粒子，并制备了含有20 wt%纳米粒子的X射线防护材料。在临床放射摄影中，研究了防护材料在40 - 100 kVp光子能量范围内的X射线衰减百分比和半价层（HVL）。使用菌落计数法评估防护材料对革兰氏阴性菌、革兰氏阳性菌和白色念珠菌的抗菌/抗真菌活性。在A549细胞上研究了防护材料的毒性。
使用含有BiO纳米粒子的防护材料获得了最高的X射线衰减百分比和最低的HVL。尽管所有防护材料都显示出抗菌活性，但含有BiO/PbO纳米粒子的防护材料在菌落计数减少方面最为有效。含有纳米BiO和BiO/PbO的两种X射线防护材料在高达500 µg/ml的浓度下对A549细胞都表现出高细胞相容性。含有PbO纳米粒子的防护材料在50 µg/ml的浓度下也具有细胞相容性。
最佳的X射线衰减性能归因于含有纳米BiO的有机硅基基质；然而，含有BiO/PbO纳米粒子的柔性防护材料可能具有成本效益且细胞相容性好，同时具有最佳的抗菌/抗真菌性能。
