Chai Jiatong, Sun Zeyu, Xing Dongyang, Zhou Qi, Xu Jiancheng
Department of Laboratory Medicine, The First Hospital of Jilin University, Changchun, China.
J Taibah Univ Med Sci. 2021 Dec 1;17(3):353-361. doi: 10.1016/j.jtumed.2021.10.011. eCollection 2022 Jun.
The establishment of reference intervals (RIs) for complement 3 (C3) and complement 4 (C4) is rare, especially by indirect methods. Therefore, this study aims to establish regional RIs for C3 and C4 by an indirect method, using relevant statistical methods.
Total of 12,313 data points for C3 and 12,125 data points for C4 were obtained from the First Hospital of Jilin University's database in China and standardised using the Tukey and Box-Cox statistical methods. The coefficients of the skewness-median-coefficient of variation curves (LMS) were used to determine the critical value for age, and a subsequent test used to compare the differences. A non-parametric method was used to establish the RIs.
The C3 and C4 concentrations showed no significant differences by sex, and a weak correlation with age. No significant difference was found after calculating the z value for the age points on the LMS curves. The RIs for C3 and C4 were 0.83-1.58 g/L and 0.15-0.40 g/L, respectively. The RIs all passed verification.
Suitable RIs for C3 and C4 were established for the local population, and will benefit clinical diagnosis. The feasibility and practicability of the indirect method were demonstrated.
补体3（C3）和补体4（C4）参考区间（RIs）的建立较为少见，尤其是通过间接方法。因此，本研究旨在采用相关统计方法通过间接方法建立C3和C4的地区参考区间。
从中国吉林大学第一医院数据库中获取了12313个C3数据点和12125个C4数据点，并使用Tukey和Box-Cox统计方法进行标准化。使用偏度-中位数-变异系数曲线（LMS）的系数来确定年龄的临界值，并使用后续检验来比较差异。采用非参数方法建立参考区间。
C3和C4浓度在性别上无显著差异，与年龄呈弱相关。计算LMS曲线上年龄点的z值后未发现显著差异。C3和C4的参考区间分别为0.83-1.58 g/L和0.15-0.40 g/L。所有参考区间均通过验证。
为当地人群建立了合适的C3和C4参考区间，将有利于临床诊断。证明了间接方法的可行性和实用性。
