Xu Shiqiang, Zhang Xiangxiang, Xu Kai, Wang Zhanqi, Zhou Xueping, Jiang Lei, Jiang Tong
Department of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, China.
Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, College of Life Sciences, Huzhou University, Huzhou, China.
Front Microbiol. 2022 May 26;13:884044. doi: 10.3389/fmicb.2022.884044. eCollection 2022.
Chlorophyll a/b-binding protein of light-harvesting complex II type 1 like (LHC II-1L) is an essential component of photosynthesis, which mainly maintains the stability of the electron transport chain. However, how the LHC II-1L protein of (FvLHC II-1L) affects viral infection remains unclear. In this study, we demonstrated that the movement protein P1 of strawberry vein banding virus (SVBV P1) interacted with FvLHC II-1L and by bimolecular fluorescence complementation and pull-down assays. SVBV P1 was co-localized with FvLHC II-1L at the edge of epidermal cells of leaves, and FvLHC II-1L protein expression was upregulated in SVBV-infected . We also found that FvLHC II-1L effectively promoted SVBV P1 to compensate for the intercellular movement of movement-deficient potato virus X (PVX) and the systemic movement of movement-deficient cucumber mosaic virus (CMV). Transient overexpression of FvLHC II-1L and inoculation of an infectious clone of SVBV showed that the course of SVBV infection in was accelerated. Collectively, the results showed that SVBV P1 protein can interact with FvLHC II-1L protein, which in turn promotes infection by SVBV.
类光系统II型1叶绿素a/b结合蛋白类似物（LHC II-1L）是光合作用的重要组成部分，主要维持电子传递链的稳定性。然而，草莓脉带病毒（FvLHC II-1L）的LHC II-1L蛋白如何影响病毒感染仍不清楚。在本研究中，我们通过双分子荧光互补和下拉试验证明了草莓脉带病毒（SVBV P1）的运动蛋白P1与FvLHC II-1L相互作用。SVBV P1与FvLHC II-1L在叶片表皮细胞边缘共定位，并且在感染SVBV的植株中FvLHC II-1L蛋白表达上调。我们还发现FvLHC II-1L有效地促进了SVBV P1弥补运动缺陷型马铃薯X病毒（PVX）的细胞间运动以及运动缺陷型黄瓜花叶病毒（CMV）的系统运动。FvLHC II-1L的瞬时过表达和SVBV感染性克隆的接种表明，植株中SVBV感染进程加快。总体而言，结果表明SVBV P1蛋白可与FvLHC II-1L蛋白相互作用，进而促进植株被SVBV感染。
