Song Changyou, Wen Haibo, Liu Guangxiang, Ma Xueyan, Lv Guohua, Wu Ningyuan, Chen Jianxiang, Xue Miaomiao, Li Hongxia, Xu Pao
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
Front Microbiol. 2022 May 26;13:861705. doi: 10.3389/fmicb.2022.861705. eCollection 2022.
With strong demand for aquatic products, as well as a rapid decrease in global fishery resources and capture fisheries, domesticating animals to provide more high-quality proteins is meaningful for humans. Freshwater drum () is widely distributed in the wild habitats of North America. However, the research on and the feed domestication with diets composed of artificial compounds remains unclear. In this study, a 4-month feeding domestication experiment was conducted with larvae to evaluate the underlying mechanism and molecular targets responsible for alternations in the ingestion performance. The results indicated that a significant increase in the final body weight was exhibited by the feed domesticated group (DOM, 114.8 g) when compared to the group that did not ingest the feed (WT, 5.3 g) as the latest version we raised From the result, the final body weight exhibited significant increase between unfavorable with the feed (WT, 5.3 g) and feed domesticated group (DOM, 114.8 g). In addition, the enzyme activity of digestive enzymes like amylase, lipase, and trypsin was increased in DOM. Genes related to appetite and perception, such as , , and , were activated in DOM. 16s rRNA gene sequencing analysis revealed that sp. increased from 58.74% to 89.77% in DOM, which accounts for the dominant upregulated microbial community at the genus level, followed by . Analogously, , , and also accounted for the down-regulated microbes in the diversity. Transcriptome and RT-PCR analysis revealed that feed domestication significantly improved protein digestion and absorption, inhibited apoptosis by AGE-RAGE signaling, and activated extracellular matrix remodeling by relaxin signaling. Integrated analysis of the microbiome and host transcriptome revealed that mediated ingestion capacity, protein utilization, and cellular homeostasis might be the underlying mechanism under feed domestication. These results indicate and its key genes relating to food ingestion and digestion could serve as the molecular targets for feed domestication and sustainable development in .
随着对水产品的强劲需求，以及全球渔业资源和捕捞渔业的迅速减少，驯化动物以提供更多高质量蛋白质对人类来说意义重大。淡水鼓鱼广泛分布于北美的野生栖息地。然而，关于淡水鼓鱼以及用人工合成化合物组成的饲料进行驯化的研究仍不清楚。在本研究中，对淡水鼓鱼幼鱼进行了为期4个月的饲料驯化实验，以评估导致摄食性能改变的潜在机制和分子靶点。结果表明，与未摄食饲料的组（WT，5.3克）相比，饲料驯化组（DOM，114.8克）的最终体重显著增加，这是我们饲养的最新一批鱼的结果。结果显示，在不适应饲料的组（WT，5.3克）和饲料驯化组（DOM，114.8克）之间，最终体重呈现出显著增加。此外，DOM组中淀粉酶、脂肪酶和胰蛋白酶等消化酶的酶活性增加。与食欲和感知相关的基因，如[具体基因名称未给出]，在DOM组中被激活。16s rRNA基因测序分析表明，淡水鼓鱼属在DOM组中从58.74%增加到89.77%，在属水平上占主导地位且是上调的微生物群落，其次是[具体微生物名称未给出]。类似地，[其他未给出的微生物名称]在多样性方面也占下调微生物。转录组和RT-PCR分析表明，饲料驯化显著改善了蛋白质的消化和吸收，通过AGE-RAGE信号通路抑制细胞凋亡，并通过松弛素信号通路激活细胞外基质重塑。微生物组和宿主转录组的综合分析表明，介导的摄食能力、蛋白质利用和细胞内稳态可能是饲料驯化的潜在机制。这些结果表明，淡水鼓鱼及其与食物摄取和消化相关的关键基因可作为饲料驯化和淡水鼓鱼可持续发展的分子靶点。
