比较转录组分析阐明了叶面喷施吡唑醚菌酯对番茄（L.）的积极生理效应。
Comparative transcriptome analysis elucidates positive physiological effects of foliar application of pyraclostrobin on tomato ( L.).
作者信息
Mesara Sureshkumar N, Dave Kirtan P, Subramanian Ramalingam B
机构信息
Department of Biosciences, Sardar Patel University, Satellite campus, Bakrol-Vadtal road, Bakrol, Anand, Gujarat 388315 India.
Indukaka Ipcowala Centre of Interdisciplinary Studies in Science and Technology-IICISST, Sardar Patel University, Vallabh Vidyanagar, Anand, Gujarat 388120 India.
出版信息
Physiol Mol Biol Plants. 2022 May;28(5):971-986. doi: 10.1007/s12298-022-01191-7. Epub 2022 Jun 3.
UNLABELLED
Strobilurins, including pyraclostrobin have frequently been reported showing positive physiological effects in various agricultural crops apart from fungicidal activity. Present study elucidates comparative transcriptome analysis of control and pyraclostrobin treated tomato leaf and identifies metabolic pathways and key genes responsible for positive effects of pyraclostrobin on tomato. Pair-end raw reads, generated by Illumina Hi-seq platform were pre-processed and good quality reads were mapped onto tomato reference genome using HISAT2 alignment programme. Transcript assembly and quantification were performed using StringTie assembler. Differential Gene Expression analysis by DESeq2 identified 1,952 upregulated genes including genes encoding pathogenesis related proteins and 835 downregulated genes. RT-PCR study showed increase in expression of (2.5-fold), (3-fold), and (1.4-fold) genes, which are the key genes of photosynthesis, gibberellic acid synthesis, and nitrogen assimilation pathways respectively identified in KEGG pathway analysis. Pyraclostrobin treated plants showed 1.6-folds increase in plant height, 3.3-folds increase in number of leaves, and 2.8-folds increase in number of flowers. Total protein content increased 1.7, 1.4, 1.2, 1.2, and 1.4 folds at 1 day after application (DAA), 4DAA, 7DAA, 10DAA, and 13DAA respectively in treated plants. Moreover, content of phenol also increased 1.14, 1.5, 2.4, and 1.5 folds in 4DAA, 7DAA, 10DAA, and 13DAA respectively. Nitrate reductase activity increased 2-fold, 1.8-fold, 1.5-fold and 1.15-fold in 1DAA, 7DAA, 10DAA and 13DAA respectively. Carbohydrate decreased in treated plants up to 7DAA. The present study is the first report of transcriptome analysis elucidating positive physiological effects of strobilurin on plant.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12298-022-01191-7.
未标注
包括吡唑醚菌酯在内的甲氧基丙烯酸酯类杀菌剂，除了具有杀菌活性外，还经常被报道对各种农作物有积极的生理影响。本研究阐明了对照和吡唑醚菌酯处理的番茄叶片的比较转录组分析，并确定了吡唑醚菌酯对番茄产生积极影响的代谢途径和关键基因。由Illumina Hi-seq平台生成的双末端原始读数经过预处理，高质量读数使用HISAT2比对程序映射到番茄参考基因组上。使用StringTie组装器进行转录本组装和定量分析。通过DESeq2进行的差异基因表达分析确定了1952个上调基因，包括编码病程相关蛋白的基因，以及835个下调基因。逆转录聚合酶链反应（RT-PCR）研究表明，光合作用、赤霉素合成和氮同化途径的关键基因（分别在京都基因与基因组百科全书（KEGG）途径分析中确定）的表达分别增加了2.5倍、3倍和1.4倍。吡唑醚菌酯处理的植株株高增加了1.6倍，叶片数量增加了3.3倍，花朵数量增加了2.8倍。处理植株在施药后1天（DAA）、4 DAA、7 DAA、10 DAA和13 DAA时总蛋白含量分别增加了1.7倍、1.4倍、1.2倍、1.2倍和1.4倍。此外，在4 DAA、7 DAA、10 DAA和13 DAA时，酚类物质含量也分别增加了1.14倍、1.5倍、2.4倍和1.5倍。硝酸还原酶活性在1 DAA、7 DAA、10 DAA和13 DAA时分别增加了2倍、1.8倍、1.5倍和1.15倍。处理植株中的碳水化合物含量在7 DAA之前下降。本研究是阐明甲氧基丙烯酸酯类杀菌剂对植物积极生理影响的转录组分析的首次报道。
补充信息
在线版本包含可在10.1007/s12298-022-01191-7获取的补充材料。
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