Yadav Pawan K, Gupta Nisha, Verma Vivek, Gupta Aditya K
Department of Biotechnology, School of Life Sciences, Central University of Rajasthan, N.H.-8, Kishangarh, Ajmer, Rajasthan 305817 India.
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012 India.
Physiol Mol Biol Plants. 2021 Apr;27(4):713-725. doi: 10.1007/s12298-021-00976-6. Epub 2021 Apr 1.
The Heat shock proteins 90 family plays pivotal roles in root growth and plant development. Its isoforms have been identified in several plant species with transcripts presents in almost all stages of plant growth. However, its functional relevance has not been completely established. Therefore, in this study, we provide evidence about the role of in tomato () root development. Using tomato cultivars with differing root phenotypes, we have shown that transcripts are in accordance with root architecture, i.e. high rooting cultivars had more expression of as compared to low rooting cultivars. Moreover, overexpression of gene in transgenic tomato plants showed significant increase in root biomass and architecture, as evident from the analysis of fresh and dry weights of root and shoot samples, primary root length and length and number of lateral roots. The transgenic lines are also more tolerant to salinity and drought stresses. The results of the present study suggest that genetic manipulation of homologs in other crops can offer promising leads to develop plant with better root biomass and architecture and improved agronomics traits, like better water and mineral absorption, salinity and drought tolerance potential.
热休克蛋白90家族在根系生长和植物发育中起着关键作用。其异构体已在几种植物物种中得到鉴定，其转录本存在于植物生长的几乎所有阶段。然而，其功能相关性尚未完全确立。因此，在本研究中，我们提供了关于其在番茄（）根系发育中作用的证据。使用具有不同根系表型的番茄品种，我们发现其转录本与根系结构一致，即高生根品种与低生根品种相比，其表达量更高。此外，转基因番茄植株中该基因的过表达显示根生物量和根系结构显著增加，这从根和地上部样本的鲜重和干重、主根长度以及侧根长度和数量的分析中可以明显看出。转基因株系对盐胁迫和干旱胁迫也更具耐受性。本研究结果表明，对其他作物中该同源基因进行遗传操作可能为培育具有更好根生物量和根系结构以及改善农艺性状（如更好的水分和矿物质吸收、耐盐性和耐旱潜力）的植物提供有前景的线索。
