Li Weitian, Wang Zixu, Cao Jing, Dong Yulan, Chen Yaoxing
Neurobiology Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Curr Issues Mol Biol. 2022 Jan 25;44(2):609-625. doi: 10.3390/cimb44020042.
Lack of sleep time is a menace to modern people, and it leads to chronic diseases and mental illnesses. Circadian processes control sleep, but little is known about how sleep affects the circadian system. Therefore, we performed a 28-day sleep restriction (SR) treatment in mice. Sleep restriction disrupted the clock genes' circadian rhythm. The circadian rhythms of the and genes disappeared. The acrophase of the clock genes (, and ) that still had a circadian rhythm was advanced, while the acrophase of negative clock gene was delayed. Clock genes' upstream signals ERK and EIFs also had circadian rhythm disorders. Accompanied by changes in the central oscillator, the plasma output signal (melatonin, corticosterone, IL-6, and TNF-α) had an advanced acrophase. While the melatonin mesor was decreased, the corticosterone, IL-6, and TNF-α mesor was increased. Our results indicated that chronic sleep loss could disrupt the circadian rhythm of the central clock through ERK and EIFs and affect the output signal downstream of the core biological clock.
睡眠时间不足对现代人来说是一种威胁，它会导致慢性疾病和精神疾病。昼夜节律过程控制睡眠，但关于睡眠如何影响昼夜节律系统却知之甚少。因此，我们对小鼠进行了为期28天的睡眠限制（SR）治疗。睡眠限制扰乱了时钟基因的昼夜节律。 和 基因的昼夜节律消失。仍具有昼夜节律的时钟基因（ 、 和 ）的峰相位提前，而负性时钟基因 的峰相位延迟。时钟基因的上游信号ERK和EIFs也出现了昼夜节律紊乱。伴随着中央振荡器的变化，血浆输出信号（褪黑素、皮质酮、IL-6和TNF-α）的峰相位提前。虽然褪黑素的中值降低，但皮质酮、IL-6和TNF-α的中值升高。我们的结果表明，长期睡眠不足可通过ERK和EIFs扰乱中央时钟的昼夜节律，并影响核心生物钟下游的输出信号。
