Atack J R, Perry E K, Bonham J R, Perry R H
J Neurochem. 1987 Jun;48(6):1845-50. doi: 10.1111/j.1471-4159.1987.tb05746.x.
The measurement of cholinesterase activities in either plasma or cerebrospinal fluid (CSF) may ultimately prove to be relevant in the diagnosis of neurological and neuropsychiatric disorders. However, studies to date have examined only total enzyme activities. Therefore in the present study we have examined the distribution of the individual molecular forms of both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in plasma and CSF using sucrose density gradient centrifugation. Although the total activities of AChE were of the same order of magnitude in plasma and CSF, there was a considerable difference (120-500-fold) between total BChE activity in the CSF and the BChE-rich plasma. The analysis of the individual molecular forms revealed that the predominant molecular species of AChE and BChE in the CSF--both lumbar and ventricular--was the G4 form. The G4 form also constituted the majority of the plasma BChE activity and, on average, over half (56%) of the plasma AChE activity. The significance of the AChE and BChE molecular form compositions of both plasma and CSF and their possible relationship to pathological states are discussed.
测量血浆或脑脊液（CSF）中的胆碱酯酶活性最终可能被证明与神经和神经精神疾病的诊断相关。然而，迄今为止的研究仅检测了总酶活性。因此，在本研究中，我们使用蔗糖密度梯度离心法检测了血浆和脑脊液中乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的各个分子形式的分布。尽管血浆和脑脊液中AChE的总活性处于相同数量级，但脑脊液中的总BChE活性与富含BChE的血浆之间存在相当大的差异（120 - 500倍）。对各个分子形式的分析表明，脑脊液（腰椎和脑室）中AChE和BChE的主要分子种类是G4形式。G4形式也构成了血浆BChE活性的大部分，平均而言，超过一半（56%）的血浆AChE活性也是G4形式。本文讨论了血浆和脑脊液中AChE和BChE分子形式组成的意义及其与病理状态的可能关系。
