Sine J P, Ferrand R, Colas B
Laboratoire de Biochimie II, Centre de Recherche de Biologie et Physico-Chimie Cellulaires, Nantes, France.
Mol Cell Biochem. 1990 Aug 10;96(2):143-51. doi: 10.1007/BF00420906.
The mucosal cells of the chicken intestine contain a cholinesterase activity essentially due to butyrylcholinesterase. The enzyme is present during embryonic and post-hatching development. The activity reaches a maximum value at day 19 in ovo and decreases prior to and after hatching up to day 4 ex ovo. Then the activity again rises reaching a second maximum at 2-3 weeks. Beyond this stage, the activity slowly decreases leveling off to the value determined in adult chicken. The enzyme exists as two globular forms (G1 and G4) soluble at low-ionic strengths. The G4 form is predominant in ovo up to day 19. From this stage and after hatching the G1 form is the main one. This change in the form proportion differentiates the mucosal cell butyrylcholinesterase from butyrylcholinesterase of other origins such as the chicken plasma enzyme which always shows a predominant G4 form.
鸡肠道的黏膜细胞含有一种胆碱酯酶活性，主要归因于丁酰胆碱酯酶。该酶在胚胎期和孵化后发育过程中均存在。其活性在胚胎发育第19天达到最大值，在孵化前和孵化后至出壳后第4天会下降。然后活性再次上升，在2至3周时达到第二个最大值。在此阶段之后，活性缓慢下降，逐渐趋于成年鸡所测定的值。该酶以两种球状形式（G1和G4）存在，在低离子强度下可溶。直到第19天，G4形式在胚胎中占主导地位。从这个阶段及孵化后，G1形式成为主要形式。这种形式比例的变化使黏膜细胞丁酰胆碱酯酶与其他来源的丁酰胆碱酯酶区分开来，例如鸡血浆酶，其始终显示出占主导地位的G4形式。
