Wheeler T T, Jordan T W, Ford H C
J Neurol Sci. 1987 Mar;78(1):87-92. doi: 10.1016/0022-510x(87)90081-5.
A double-label two-dimensional electrophoretic procedure has been used to search for abnormal proteins in the serum and plasma of patients with definite multiple sclerosis. The procedure possesses high resolving power and is particularly valuable in comparative studies of complex mixtures of proteins since differences due to gel-to-gel variations in protein mobilities are eliminated. Proteins present in serum and plasma at a concentration of 5-10 micrograms/ml are detected routinely. No consistent differences were observed between patients with multiple sclerosis and normal subjects in comparisons using pooled or individual specimens of serum or plasma. The absence of consistent differences in serum and plasma proteins between normal subjects and MS patients applied even when the sensitivity of the procedure was increased several-fold and when the possibly obscuring effect of albumin in the electrophoretic gels was eliminated. To our knowledge, this study combines the most extensive and sensitive search for consistent abnormalities in serum and plasma proteins in multiple sclerosis sera thus far reported.
一种双标记二维电泳方法已被用于在确诊的多发性硬化症患者的血清和血浆中寻找异常蛋白质。该方法具有高分辨率，在蛋白质复杂混合物的比较研究中特别有价值，因为消除了凝胶间蛋白质迁移率变化所导致的差异。血清和血浆中浓度为5 - 10微克/毫升的蛋白质可常规检测到。在使用合并或个体血清或血浆样本进行比较时，多发性硬化症患者与正常受试者之间未观察到一致的差异。即使该方法的灵敏度提高了几倍，并且消除了电泳凝胶中白蛋白可能产生的遮蔽效应，正常受试者与多发性硬化症患者之间血清和血浆蛋白质仍未出现一致的差异。据我们所知，本研究是迄今为止对多发性硬化症血清中血清和血浆蛋白质一致性异常进行的最广泛、最灵敏的搜索。
