Wu J Y, Welch K M, Duncan C C
Neurochem Res. 1979 Dec;4(6):811-20. doi: 10.1007/BF00964477.
Sera from patients with multiple sclerosis (MS) and those obtained from normal subjects are indistinguishable by regular 5% or 7% polyacrylamide gel electrophoresis. However, 11 out of 15 MS sera examined by gradient polyacrylamide gel electrophoresis showed three distinct protein bands. None of the sera obtained from 10 normal subjects showed the characteristic protein patterns when they were examined by gradient gel electrophoresis. Similar results were obtained with de-albumin serum samples or with serum proteins precipitable at 50% ammonium sulfate saturation. These three proteins have now been purified to homogeneity by preparative gradient gel electrophoresis. Molecular weights of these proteins were estimated from gradient gel electrophoresis as 398,000, 363,000 and 302,000 daltons, respectively.
多发性硬化症（MS）患者的血清与正常受试者的血清，通过常规5%或7%的聚丙烯酰胺凝胶电泳无法区分。然而，在通过梯度聚丙烯酰胺凝胶电泳检测的15份MS血清中，有11份显示出三条不同的蛋白带。当通过梯度凝胶电泳检测时，从10名正常受试者获得的血清均未显示出特征性的蛋白模式。用脱白蛋白血清样本或在50%硫酸铵饱和度下可沉淀的血清蛋白也得到了类似结果。现已通过制备性梯度凝胶电泳将这三种蛋白纯化至均一状态。通过梯度凝胶电泳估计，这些蛋白的分子量分别为398,000、363,000和302,000道尔顿。
