Magrassi L, Purves D, Lichtman J W
J Neurosci. 1987 Apr;7(4):1207-14. doi: 10.1523/JNEUROSCI.07-04-01207.1987.
We have evaluated the efficacy of 18 cationic mitochondrial dyes that, as a class, show some ability to stain living nerve terminals. Several of these agents provide excellent staining of neuromuscular junctions in a wide range of species. More detailed studies of the most effective of these dyes--4-(4-diethylaminostyryl)-N-methylpyridinium iodide (4-Di-2-ASP)--indicate that it has no lasting effect on the structure or function of motor nerve terminals. As demonstrated in the accompanying paper (Lichtman et al., 1987; see also Lichtman et al., 1986), 4-Di-2-ASP can therefore be used to follow the configuration of identified motor terminals over arbitrarily long intervals.
我们评估了18种阳离子线粒体染料的功效，作为一类染料，它们都具有一定的能力来对活神经末梢进行染色。其中几种试剂能对多种物种的神经肌肉接头进行出色的染色。对这些染料中最有效的——4-(4-二乙氨基苯乙烯基)-N-甲基碘化吡啶鎓（4-Di-2-ASP）——进行的更详细研究表明，它对运动神经末梢的结构或功能没有持久影响。正如随附论文（Lichtman等人，1987年；另见Lichtman等人，1986年）所示，因此4-Di-2-ASP可用于在任意长的时间间隔内追踪已识别运动末梢的形态。
